HLA-A, -B, -C genotyping and expression in human nonlymphoid tumor cell lines

Laura Delfino, Gina Ciccarelli, Davide Bini, Anna Morabito, Sarah Pozzi, Aline Martayan, Ezio Giorda, Andrea Setini, Rocco Fraioli, Patrizio Giacomini, Giovan Battista Ferrara

Research output: Contribution to journalArticle

Abstract

A combination of molecular genotyping and protein biochemistry methods was used to assess the HLA-A, -B, -C genotyping and expression of six tumor cell lines. Four cell lines had been previously HLA typed by conventional serologic methods. Two could not be typed by serology because deficient in the surface expression of HLA-A, -B, -C molecules. As shown herein, all the 25 alleles carried by the six tested cell lines were typed at the DNA level. In addition, discrepancies between the previous serologic and the present DNA typing results were detected in 9 of the 21 tested serologic specificities. Typing at the protein level by isoelectric focusing and allele-specific monoclonal antibodies confirmed the DNA typing data. Our results exemplify the limits of the serologic typing procedures and demonstrate that molecular methods are highly desirable to conduct functional experiments and identify HLA losses in neoplastic cells at single allele level.

Original languageEnglish
Pages (from-to)7-15
Number of pages9
JournalJournal of Immunotherapy
Volume22
Issue number1
DOIs
Publication statusPublished - 1999

Keywords

  • HLA typing
  • HLA-A
  • HLA-B
  • HLA-C
  • IEF
  • PCR

ASJC Scopus subject areas

  • Cancer Research
  • Pharmacology
  • Immunology

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    Delfino, L., Ciccarelli, G., Bini, D., Morabito, A., Pozzi, S., Martayan, A., Giorda, E., Setini, A., Fraioli, R., Giacomini, P., & Ferrara, G. B. (1999). HLA-A, -B, -C genotyping and expression in human nonlymphoid tumor cell lines. Journal of Immunotherapy, 22(1), 7-15. https://doi.org/10.1097/00002371-199901000-00002