HLA-A*02 subtype distribution in Caucasians from northern Italy: Identification of A*0220

K. Fleischhauer, E. Zino, B. Mazzi, G. M. Severini, E. Benazzi, C. Bordignon

Research output: Contribution to journalArticlepeer-review

Abstract

This study describes a comprehensive, easy to perform PCR-SSOP typing approach suitable for complete genomic subtyping of HLA-A*02. A single 1.6 kb PCR-amplificate spanning exons 2, 3 and 4 of the HLA-A*02 gene was used for hybridization with a panel of twenty-four SSOPs. This allowed unequivocal assignment of all so far known HLA-A2 subtypes, including A*0209 and A*0215N which differ for nucleotide substitutions in exon 4, without the need for two separate amplifications. Using this approach, HLA-A*02 subtype distribution was analyzed in 218 samples from unrelated, healthy individuals from northern Italy enrolled in the Italian Bone Marrow Registry and typed as HLA-A2 by serology or generic molecular analysis. As expected, A*0201 was found in the majority (92.6%) of samples. However, a significant number (6.8%) of individuals carried A':0205. Furthermore, A'0202, A'0208, A'0209 and A'0217, so far not described in Caucasians, were detected in a low number of samples (frequency ranging from 0.45% to 1.8%). Finally, a novel HLA-A'02 subtype, A'0220, was detected in 0.9% of the samples. As confirmed by DNA sequencing of exons 2 and 3, this allele is identical to A'0201 except for a single nucleotide substitution in codon 66 which changes the predicted amino acid sequence form Lys to Asn. The findings of this study have implications for the selection of HLA-A'02' donors in unrelated bone marrow transplantation and of patients for specific immunetherapy with HLA-A'02 restricted peptide vaccines.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalTissue Antigens
Volume48
Issue number6
Publication statusPublished - 1996

Keywords

  • A*0220
  • HLA-A2 subtypes
  • PCR-SSOP
  • Population study

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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