Hla-a3-restricted ctl defined antigens shared among human melanomas but not melanocytes

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Abstract

Anti-melanoma T cell lines and clones were isolated from tumor-involved or tumor-free lymph nodes (TAL, tumor-associated lymphocytes and LNL, lymph node lymphocytes, respectively) of two HLA-A3.1 + melanoma patients (4473 and 15329). Two CTL lines obtained from LNL of both patients (LNL 4473 and LNL 15392). two indipendent CTL lines derived from TAL 15392 (245 LDA and 249.1) and a CTL clone derived from LNL 15392 (13B5) were studied. Blocking experiments of autologous tumor lysis showed that these effectors recognized a melanoma antigen in the context of HLA-A3 allele. Analysis of specificity performed on HLA-A3+ and HLA- A3- normal or neo plastic cells of the melanocyte lineage indicated that LNL 4473, 245 LDA, 13B5. but not 249.1, recognized common melanoma antigens, not expressed on melanocytes, in the context of HLA-A3 molecule. LNL 15392 showed the same recognition pattern of 13B5 on HLA-A3+ melanomas but, in addition. LNL 15392 lysed an aliogeneic HLA-A3- melanoma sharing HLA-B14 with thy autologous melanoma. No reactivity was seen by these effectors on aliogeneic HLA-A3+ tumor targets of nonrnelanocyte lineage. COS-7 cells transiently transfected with HLA-A3 and most of the genes known to code for melanoma-associated antigens suggested that our CTLs recognized new melanoma antigens. Finally, epitope reconstitution experiments with naturally processed peptides of Me 15329 indicated that the HPLC fractions 47-51 contained the antigenic peptides seen by LNL 15392 in the context of HLA-A3 molecule. Further studies will be performed to identify the melanoma anti-tfens recognized by the HLA-A3-restricted anti-melanoma CTLs.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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