HLA-B35 Influences the Apoptosis Rate in Human Peripheral Blood Mononucleated Cells and HLA-Transfected Cells

Giulia Salazar, Gualtiero Colombo, Stefania Lenna, Rita Antonioli, Lorenzo Beretta, Alessandro Santaniello, Raffaella Scorza

Research output: Contribution to journalArticle

Abstract

Human leukocyte antigen (HLA) class I antigens can act as signal-transducing molecules that influence individual reactivity to external stimuli and the existence of haplotype-specific cell signal regulation has been suggested. In this article, we provide definite experimental evidence for the existence of a HLA-B35 haplotype-specific regulation of cell apoptosis in different experimental models. First, we demonstrated that HLA-B35, but not other HLA-class I antigens, was associated with an increased cell susceptibility to apoptosis in human peripheral mononuclear cells (PBMCs) exposed in vitro to thapsigargin. Second, we confirmed this association in human ECV 304 cells transfected with HLA-B35 or with HLA-B8, an antigen that did not appear to influence the apoptosis rate in the thapsigargin-treated PBMCs. Third, we confirmed the specific influence of HLA-B35 on cell apoptosis in non human cells (i.e., HLA-B35-transfected NIH3T3 murine fibroblasts). Our data show the existence of HLA-B35 haplotype-specific regulation of cell apoptosis and open new perspectives on the role of HLA class I genes in cell activation and disease susceptibility.

Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalHuman Immunology
Volume68
Issue number3
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Apoptosis
  • ECV304
  • HLA class I
  • HLA-B35
  • NIH3T3
  • PBMC
  • Taxol
  • thapsigargin

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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