ESPRESSIONE DI MOLECOLE HLA DI CLASSE I (A, B, C) E DI CLASSE II (DR) SU OSTEOBLASTI UMANI COLTIVATI IN VITRO

Translated title of the contribution: HLA class I (A, B, C) and class II (DR) antigen expression by in vitro cultured human osteoblast-like bone cells

P. Manduca, A. Leprini, A. Federici, F. Sanguineti, M. Costantini, E. Stringa, S. Barocci, A. Nocera

Research output: Contribution to journalArticlepeer-review

Abstract

Human osteoblast-like cell lines were established in vitro from spongious bone fragments and analyzed by means of indirect immunoperoxidase technique for the expression of HLA class I (A, B, C) and class II (DR) antigens when cultured in absence or after culture in presence of human recombinant cytokines such as IL-1, IL-6, tumor necrosis factor-(TNF-α) and interferon-γ(IFN-γ). The results showed that: 1) osteoblast-like bone cells, studied after the third and the fifth in vitro passage, appeared positive for IILA class I antigens but did not express HLA-DR molecules. 2) Following in vitro culture in the presence of the above cytokins, TNF-α and IFN-γ only were able both to upregulate HLA-class I expression and to induce DR antigens on osteoblast cells. The above data may have some importance for the understanding of immune mechanisms regulation bone resorption at the bone remodeling sites.

Translated title of the contributionHLA class I (A, B, C) and class II (DR) antigen expression by in vitro cultured human osteoblast-like bone cells
Original languageItalian
Pages (from-to)241-244
Number of pages4
JournalMinerva Ortopedica e Traumatologica
Volume43
Issue number4
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Surgery

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