Human osteoblast-like cell lines were established in vitro from spongious bone fragments and analyzed by means of indirect immunoperoxidase technique for the expression of HLA class I (A, B, C) and class II (DR) antigens when cultured in absence or after culture in presence of human recombinant cytokines such as IL-1, IL-6, tumor necrosis factor-(TNF-α) and interferon-γ(IFN-γ). The results showed that: 1) osteoblast-like bone cells, studied after the third and the fifth in vitro passage, appeared positive for IILA class I antigens but did not express HLA-DR molecules. 2) Following in vitro culture in the presence of the above cytokins, TNF-α and IFN-γ only were able both to upregulate HLA-class I expression and to induce DR antigens on osteoblast cells. The above data may have some importance for the understanding of immune mechanisms regulation bone resorption at the bone remodeling sites.
|Translated title of the contribution||HLA class I (A, B, C) and class II (DR) antigen expression by in vitro cultured human osteoblast-like bone cells|
|Number of pages||4|
|Journal||Minerva Ortopedica e Traumatologica|
|Publication status||Published - 1992|
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