HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term

G. Terrazzano, M. F. Romano, M. C. Turco, S. Salzano, A. Ottaiano, S. Venuta, S. Fontana, C. Manzo, S. Zappacosta, Ennio Carbone

Research output: Contribution to journalArticlepeer-review

Abstract

The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.

Original languageEnglish
Pages (from-to)326-332
Number of pages7
JournalTissue Antigens
Volume55
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • HLA class I antigens
  • IL-10
  • NF-κB factors

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

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