TY - JOUR
T1 - HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term
AU - Terrazzano, G.
AU - Romano, M. F.
AU - Turco, M. C.
AU - Salzano, S.
AU - Ottaiano, A.
AU - Venuta, S.
AU - Fontana, S.
AU - Manzo, C.
AU - Zappacosta, S.
AU - Carbone, Ennio
PY - 2000
Y1 - 2000
N2 - The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.
AB - The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.
KW - HLA class I antigens
KW - IL-10
KW - NF-κB factors
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U2 - 10.1034/j.1399-0039.2000.550406.x
DO - 10.1034/j.1399-0039.2000.550406.x
M3 - Article
C2 - 10852384
AN - SCOPUS:0034011310
VL - 55
SP - 326
EP - 332
JO - Tissue Antigens
JF - Tissue Antigens
SN - 0001-2815
IS - 4
ER -