HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term

G. Terrazzano, M. F. Romano, M. C. Turco, S. Salzano, A. Ottaiano, S. Venuta, S. Fontana, C. Manzo, S. Zappacosta, Ennio Carbone

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.

Original languageEnglish
Pages (from-to)326-332
Number of pages7
JournalTissue Antigens
Volume55
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

Histocompatibility Antigens Class I
Antigen Presentation
HLA Antigens
Interleukin-10
Down-Regulation
Antigens
Cells
Cell Line
Molecules
Interleukins
Major Histocompatibility Complex
Transfection
Transcription Factors
Genes

Keywords

  • HLA class I antigens
  • IL-10
  • NF-κB factors

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term. / Terrazzano, G.; Romano, M. F.; Turco, M. C.; Salzano, S.; Ottaiano, A.; Venuta, S.; Fontana, S.; Manzo, C.; Zappacosta, S.; Carbone, Ennio.

In: Tissue Antigens, Vol. 55, No. 4, 2000, p. 326-332.

Research output: Contribution to journalArticle

Terrazzano, G, Romano, MF, Turco, MC, Salzano, S, Ottaiano, A, Venuta, S, Fontana, S, Manzo, C, Zappacosta, S & Carbone, E 2000, 'HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term', Tissue Antigens, vol. 55, no. 4, pp. 326-332. https://doi.org/10.1034/j.1399-0039.2000.550406.x
Terrazzano, G. ; Romano, M. F. ; Turco, M. C. ; Salzano, S. ; Ottaiano, A. ; Venuta, S. ; Fontana, S. ; Manzo, C. ; Zappacosta, S. ; Carbone, Ennio. / HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term. In: Tissue Antigens. 2000 ; Vol. 55, No. 4. pp. 326-332.
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