Abstract
The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.
| Original language | English |
|---|---|
| Pages (from-to) | 326-332 |
| Number of pages | 7 |
| Journal | Tissue Antigens |
| Volume | 55 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2000 |
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Keywords
- HLA class I antigens
- IL-10
- NF-κB factors
ASJC Scopus subject areas
- Immunology
- Cell Biology
Cite this
HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term. / Terrazzano, G.; Romano, M. F.; Turco, M. C.; Salzano, S.; Ottaiano, A.; Venuta, S.; Fontana, S.; Manzo, C.; Zappacosta, S.; Carbone, Ennio.
In: Tissue Antigens, Vol. 55, No. 4, 2000, p. 326-332.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HLA class I antigen downregulation by interleukin (IL)-10 is predominantly governed by NF-κB in the short term and by TAP1 + 2 in the long term
AU - Terrazzano, G.
AU - Romano, M. F.
AU - Turco, M. C.
AU - Salzano, S.
AU - Ottaiano, A.
AU - Venuta, S.
AU - Fontana, S.
AU - Manzo, C.
AU - Zappacosta, S.
AU - Carbone, Ennio
PY - 2000
Y1 - 2000
N2 - The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.
AB - The present study was designed to determine the molecular mechanisms by which interleukin (IL-10) prevents the HLA class I antigen expression at the cell surface. In this context, the potential role of transporter associated with antigen presentation 1 + 2 (TAP1 + 2) molecules and NF-κB transcription factors was addressed. The IL-10 effect was investigated in a human lymphoblastoid cell system defective for TAP1 + 2 genes (T2 cell line) and in the related TAP1 +2 transfectants (T3 cell line). In this experimental system, after 48 h of incubation in the presence of IL-10, the HLA class I antigen downmodulalion was observed in the T3 but not in the T2 cell line, suggesting a potential role of TAP1 +2 molecules. In the same experimental conditions, the NF-κB activity was unaffected. Instead, after 3 h of exposure to IL-10, the HLA downmodulation was observed in both cell lines, the NF-κB factors activity being strongly reduced. In addition, the transfection of the inhibitor of NF-κB, IκBα, prevented the IL-10 effect on HLA class I antigen expression in the T3 cell line. This phenomenon was observed after 3 h but not 48 h of IL-10 incubation. These evidences indicate a time dependent involvement of TAP1 + 2 antigens and of NF-κB activity in the IL-10-induced major histocompatibility complex (MHC) class I downmodulation.
KW - HLA class I antigens
KW - IL-10
KW - NF-κB factors
UR - http://www.scopus.com/inward/record.url?scp=0034011310&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034011310&partnerID=8YFLogxK
U2 - 10.1034/j.1399-0039.2000.550406.x
DO - 10.1034/j.1399-0039.2000.550406.x
M3 - Article
C2 - 10852384
AN - SCOPUS:0034011310
VL - 55
SP - 326
EP - 332
JO - Tissue Antigens
JF - Tissue Antigens
SN - 0001-2815
IS - 4
ER -