HLA-class I-specific inhibitory receptors in human cytolytic T lymphocytes

Molecular characterization, distribution in lymphoid tissues and co-expression by individual T cells

Maria Cristina Mingari, Marco Ponte, Claudia Cantoni, Chiara Vitale, Francesca Schiavetti, Stefanie Bertone, Rosanna Bellomo, Andrea Tradori Cappai, Roberto Biassoni

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A subset of cytolytic T lymphocytes has been shown to express receptors of the NK type (NKR) which can inhibit T cell cytotoxicity induced via the TCR-CD3 pathway. In this study, by the analysis of full length cDNA amplified from representative T cell clones, we show that NKR belonging either to the Ig superfamily, including p58.1, p58.2, p70 and p140, or to the C-type lectin superfamily (CD94/NKG2A), display sequences which are identical to those of the corresponding NKR expressed by CD3- NK cells. Moreover, a fragment of cDNA encoding the NKG2A protein was consistently amplified from all CD94+ T cell clones analyzed. Since different NKR types can be expressed by T cells, we analyzed whether individual T cells could cc-express more than one NKR. Analysis of either resting or activated (and cultured) T cell populations revealed that two or more NKR can be co-expressed by single T cells. Moreover, by the analysis of T cell clones, we show that co-expressed receptors are functional and can inhibit independently the TCR-induced cytolytic function. Finally, we investigated whether NKR+ T lymphocytes were also present in lymphoid tissues. No such cells were found in thymus or cord blood, thus further supporting the notion that they represent memory T cells. On the other hand, they were present in all the peripheral tissues analyzed including spleen, lymph nodes and tonsils.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalInternational Immunology
Volume9
Issue number4
DOIs
Publication statusPublished - 1997

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Lymphoid Tissue
T-Lymphocytes
Clone Cells
Complementary DNA
C-Type Lectins
Palatine Tonsil
T-Lymphocyte Subsets
Fetal Blood
Natural Killer Cells
Thymus Gland
Cultured Cells
Spleen
Lymph Nodes

Keywords

  • Cytolytic T lymphocytes
  • HLA class I molecules
  • Inhibitory receptors
  • NK cells

ASJC Scopus subject areas

  • Immunology

Cite this

HLA-class I-specific inhibitory receptors in human cytolytic T lymphocytes : Molecular characterization, distribution in lymphoid tissues and co-expression by individual T cells. / Mingari, Maria Cristina; Ponte, Marco; Cantoni, Claudia; Vitale, Chiara; Schiavetti, Francesca; Bertone, Stefanie; Bellomo, Rosanna; Cappai, Andrea Tradori; Biassoni, Roberto.

In: International Immunology, Vol. 9, No. 4, 1997, p. 485-491.

Research output: Contribution to journalArticle

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AU - Vitale, Chiara

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