HLA class II alleles associations of anticardiolipin and anti-β2GPI antibodies in a large series of European patients with systemic lupus erythematosus

Mauro Galeazzi, Gian Domenico Sebastiani, Angela Tincani, Jean Charles Piette, Flavio Allegri, Gabriella Morozzi, Francesca Bellisai, Raffaella Scorza, Giovanni Battista Ferrara, Carlo Carcassi, Josep Font, Giuseppe Passiu, Josef Smolen, Chryssa Papasteriades, Frederic Houssiau, Antonio Fernandez Nebro, Enrique De Ramon Garrido, Anna Jedryka-Goral, Roberto Marcolongo

Research output: Contribution to journalArticle

Abstract

The objective of this study was to determine the HLA class II associations of the anticardiolipin (aCL) and anti-β2GPI (aβ2GPI) antibodies in a large series of European patients with systemic lupus erythematosus (SLE). A cohort of 577 European SLE patients was enrolled. aCL and aβ2GPI were measured by ELISA methods. Molecular typing of HLA-DRB1, DRB3, DRB4, DRB5, DQA1 and DQB1 loci was performed by the polymerase chain reaction sequence specific oligonucleotide probes (PCR SSOP) method. aCL of IgG, IgM and IgA isotypes were detected in 22.8%, 14% and 13.9% of patients, respectively. IgG and IgM aβ2GPI were detected in 20% of patients. aCL showed positive association with HLA DRB1*04, DRB1*0402, DRB1*0403, DRB1*07, DRB3*0301, DQA1*0201, DQA1*0301, DQB1*0302, and negative association with DQA1*0501, DRB3*0202. aβ2GPI showed positive association with DRB1*0402, DRB1*0403, DQB1*0302.. DRB1*0402 carried the highest relative risk for the presence of both aCL (RR = 8.1) and aβ2GPI (RR = 4.6). Our results confirm the already described associations of aCL with HLA DR4 and DR7, but also demonstrate that, among the alleles at the DRB1*04 locus, the *0402 was most represented both in aCL and in aβ2GPI positive patients. In addition, HLA class II associations of aβ2GPI are for the first time extensively examined in a large cohort of European SLE patients.

Original languageEnglish
Pages (from-to)47-55
Number of pages9
JournalLupus
Volume9
Issue number1
Publication statusPublished - 2000

Keywords

  • Antiβ2GPI antibodies
  • Anticardiolipin antibodies
  • HLA class II
  • SLE

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

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    Galeazzi, M., Sebastiani, G. D., Tincani, A., Piette, J. C., Allegri, F., Morozzi, G., Bellisai, F., Scorza, R., Ferrara, G. B., Carcassi, C., Font, J., Passiu, G., Smolen, J., Papasteriades, C., Houssiau, F., Nebro, A. F., De Ramon Garrido, E., Jedryka-Goral, A., & Marcolongo, R. (2000). HLA class II alleles associations of anticardiolipin and anti-β2GPI antibodies in a large series of European patients with systemic lupus erythematosus. Lupus, 9(1), 47-55.