HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia

C Tersigni, C W Redman, R Dragovic, D Tannetta, G Scambia, N Di Simone, I Sargent, M Vatish

Research output: Contribution to journalArticle

Abstract

In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalJournal of Reproductive Immunology
Volume129
DOIs
Publication statusPublished - Sep 2018

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Trophoblasts
HLA-DR Antigens
Pre-Eclampsia
Mothers
Placenta
HLA-G Antigens
HLA-C Antigens
Flow Cytometry
Perfusion
Immunohistochemistry
Biopsy
Pregnancy

Cite this

Tersigni, C., Redman, C. W., Dragovic, R., Tannetta, D., Scambia, G., Di Simone, N., ... Vatish, M. (2018). HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia. Journal of Reproductive Immunology, 129, 48-52. https://doi.org/10.1016/j.jri.2018.06.024

HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia. / Tersigni, C; Redman, C W; Dragovic, R; Tannetta, D; Scambia, G; Di Simone, N; Sargent, I; Vatish, M.

In: Journal of Reproductive Immunology, Vol. 129, 09.2018, p. 48-52.

Research output: Contribution to journalArticle

Tersigni, C, Redman, CW, Dragovic, R, Tannetta, D, Scambia, G, Di Simone, N, Sargent, I & Vatish, M 2018, 'HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia', Journal of Reproductive Immunology, vol. 129, pp. 48-52. https://doi.org/10.1016/j.jri.2018.06.024
Tersigni, C ; Redman, C W ; Dragovic, R ; Tannetta, D ; Scambia, G ; Di Simone, N ; Sargent, I ; Vatish, M. / HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia. In: Journal of Reproductive Immunology. 2018 ; Vol. 129. pp. 48-52.
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AU - Scambia, G

AU - Di Simone, N

AU - Sargent, I

AU - Vatish, M

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N2 - In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.

AB - In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.

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