HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression

Pascale Paul, Nathalie Rouas-Freiss, Philippe Moreau, Francisco Adrian Cabestre, Catherine Menier, Iman Khalil-Daher, Céline Pangault, Myriam Onno, Renée Fauchet, Jorge Martinez-Laso, Pablo Morales, Antonio Arnaiz Villena, Patrizio Giacomini, Pier Giorgio Natali, Guido Frumento, Giovan Battista Ferrara, Michael McMaster, Susan Fisher, Danny Schust, Soldano FerroneJean Dausset, Dan Geraghty, Edgardo D. Carosella

Research output: Contribution to journalArticle

Abstract

Non-classical MHC class I HLA-E, -F, and -G molecules differ from classical class I histocompatibility antigens by specific patterns of transcription, protein expression, and immunological functions. Restriction of the expression pattern of these non-classical antigens may play a key role in modulation of immune responses during pregnancy and diseases but remains to be additionally defined. A specific component of the second International Conference on HLA-G and the 13th HLA-G Histocompatibility Workshop will be dedicated to the analysis of transcription and expression of non-classical class I genes in normal and pathological tissues. In a first step, referred to as the preworkshop, we here report the analysis and conclusions of a working group which was constituted to gather and validate optimal reagents and protocols allowing RT-PCR analysis of HLA-E, -F, -G transcript levels and flow cytometry and immunochemistry analysis of HLA-G expression in cells and tissues. As a result of this work, use of specific primers and probes detecting alternative transcripts of HLA-E, -F, and G have been validated in transfected cells expressing differential pattern of HLA class I antigens. Analysis of the specificity and affinity of collected antibodies has allowed definition of reagents to be proposed for immunochemistry and flow cytometry analysis of HLA-G expression in normal and pathological tissues during the workshop. This work has allowed constitution of an extended workshop group which is now initiating analysis of non-classical class I transcription and expression in various cells and tissues, a collective contribution that will additionally refine our view of the expression of these antigens in normal and pathological situations.

Original languageEnglish
Pages (from-to)1177-1195
Number of pages19
JournalHuman Immunology
Volume61
Issue number11
DOIs
Publication statusPublished - 2000

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Keywords

  • HLA-G monoclonal antibodies
  • HLA-G, -F, -E transcription
  • Non-classical HLA class I genes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Paul, P., Rouas-Freiss, N., Moreau, P., Cabestre, F. A., Menier, C., Khalil-Daher, I., Pangault, C., Onno, M., Fauchet, R., Martinez-Laso, J., Morales, P., Villena, A. A., Giacomini, P., Natali, P. G., Frumento, G., Ferrara, G. B., McMaster, M., Fisher, S., Schust, D., ... Carosella, E. D. (2000). HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression. Human Immunology, 61(11), 1177-1195. https://doi.org/10.1016/S0198-8859(00)00154-3