HLA-G, -E, -F preworkshop

Tools and protocols for analysis of non-classical class I genes transcription and protein expression

Pascale Paul, Nathalie Rouas-Freiss, Philippe Moreau, Francisco Adrian Cabestre, Catherine Menier, Iman Khalil-Daher, Céline Pangault, Myriam Onno, Renée Fauchet, Jorge Martinez-Laso, Pablo Morales, Antonio Arnaiz Villena, Patrizio Giacomini, Pier Giorgio Natali, Guido Frumento, Giovan Battista Ferrara, Michael McMaster, Susan Fisher, Danny Schust, Soldano Ferrone & 3 others Jean Dausset, Dan Geraghty, Edgardo D. Carosella

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Non-classical MHC class I HLA-E, -F, and -G molecules differ from classical class I histocompatibility antigens by specific patterns of transcription, protein expression, and immunological functions. Restriction of the expression pattern of these non-classical antigens may play a key role in modulation of immune responses during pregnancy and diseases but remains to be additionally defined. A specific component of the second International Conference on HLA-G and the 13th HLA-G Histocompatibility Workshop will be dedicated to the analysis of transcription and expression of non-classical class I genes in normal and pathological tissues. In a first step, referred to as the preworkshop, we here report the analysis and conclusions of a working group which was constituted to gather and validate optimal reagents and protocols allowing RT-PCR analysis of HLA-E, -F, -G transcript levels and flow cytometry and immunochemistry analysis of HLA-G expression in cells and tissues. As a result of this work, use of specific primers and probes detecting alternative transcripts of HLA-E, -F, and G have been validated in transfected cells expressing differential pattern of HLA class I antigens. Analysis of the specificity and affinity of collected antibodies has allowed definition of reagents to be proposed for immunochemistry and flow cytometry analysis of HLA-G expression in normal and pathological tissues during the workshop. This work has allowed constitution of an extended workshop group which is now initiating analysis of non-classical class I transcription and expression in various cells and tissues, a collective contribution that will additionally refine our view of the expression of these antigens in normal and pathological situations.

Original languageEnglish
Pages (from-to)1177-1195
Number of pages19
JournalHuman Immunology
Volume61
Issue number11
DOIs
Publication statusPublished - 2000

Fingerprint

HLA-G Antigens
MHC Class I Genes
Immunochemistry
Histocompatibility Antigens Class I
Education
Flow Cytometry
Proteins
Antigens
Histocompatibility
Antibody Affinity
Constitution and Bylaws
HLA Antigens
Pregnancy
Polymerase Chain Reaction

Keywords

  • HLA-G monoclonal antibodies
  • HLA-G, -F, -E transcription
  • Non-classical HLA class I genes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Paul, P., Rouas-Freiss, N., Moreau, P., Cabestre, F. A., Menier, C., Khalil-Daher, I., ... Carosella, E. D. (2000). HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression. Human Immunology, 61(11), 1177-1195. https://doi.org/10.1016/S0198-8859(00)00154-3

HLA-G, -E, -F preworkshop : Tools and protocols for analysis of non-classical class I genes transcription and protein expression. / Paul, Pascale; Rouas-Freiss, Nathalie; Moreau, Philippe; Cabestre, Francisco Adrian; Menier, Catherine; Khalil-Daher, Iman; Pangault, Céline; Onno, Myriam; Fauchet, Renée; Martinez-Laso, Jorge; Morales, Pablo; Villena, Antonio Arnaiz; Giacomini, Patrizio; Natali, Pier Giorgio; Frumento, Guido; Ferrara, Giovan Battista; McMaster, Michael; Fisher, Susan; Schust, Danny; Ferrone, Soldano; Dausset, Jean; Geraghty, Dan; Carosella, Edgardo D.

In: Human Immunology, Vol. 61, No. 11, 2000, p. 1177-1195.

Research output: Contribution to journalArticle

Paul, P, Rouas-Freiss, N, Moreau, P, Cabestre, FA, Menier, C, Khalil-Daher, I, Pangault, C, Onno, M, Fauchet, R, Martinez-Laso, J, Morales, P, Villena, AA, Giacomini, P, Natali, PG, Frumento, G, Ferrara, GB, McMaster, M, Fisher, S, Schust, D, Ferrone, S, Dausset, J, Geraghty, D & Carosella, ED 2000, 'HLA-G, -E, -F preworkshop: Tools and protocols for analysis of non-classical class I genes transcription and protein expression', Human Immunology, vol. 61, no. 11, pp. 1177-1195. https://doi.org/10.1016/S0198-8859(00)00154-3
Paul, Pascale ; Rouas-Freiss, Nathalie ; Moreau, Philippe ; Cabestre, Francisco Adrian ; Menier, Catherine ; Khalil-Daher, Iman ; Pangault, Céline ; Onno, Myriam ; Fauchet, Renée ; Martinez-Laso, Jorge ; Morales, Pablo ; Villena, Antonio Arnaiz ; Giacomini, Patrizio ; Natali, Pier Giorgio ; Frumento, Guido ; Ferrara, Giovan Battista ; McMaster, Michael ; Fisher, Susan ; Schust, Danny ; Ferrone, Soldano ; Dausset, Jean ; Geraghty, Dan ; Carosella, Edgardo D. / HLA-G, -E, -F preworkshop : Tools and protocols for analysis of non-classical class I genes transcription and protein expression. In: Human Immunology. 2000 ; Vol. 61, No. 11. pp. 1177-1195.
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AU - Menier, Catherine

AU - Khalil-Daher, Iman

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