HLA-G regulatory polymorphisms are associated with susceptibility to HCV infection

E. Catamo, L. Zupin, Nadia Freato, V. Polesello, F. Celsi, S. L. Crocè, Flora Masutti, Gabriele Pozzato, L. Segat, S. Crovella

Research output: Contribution to journalArticle

Abstract

Background: Hepatitis C virus (HCV) is able to bypass the immune system modulating innate and adaptive immune response and blocking T helper 1 (Th1) cell production. Because the human leukocyte antigen (HLA)-G molecule has immunomodulatory properties inhibiting the function and production of natural killer and cytotoxic lymphocyte T cells, as well as promoting shift from Th1 toward Th2 response, we hypothesized its involvement in susceptibility to HCV infection. Materials and Methods: Considering that HLA-G mRNA expression has been reported to be under genetic control, an association study was conducted analyzing 800 base pairs upstream the ATG at the 5′upstream regulator region (URR) and 850 base pairs from ATG to exon 3 and the 3′untranslated region (UTR) of HLA-G gene in Italian HCV-positive patients and uninfected controls. Results: Four 5′URR polymorphisms (−725C>G>T, −509C>G, −400G>A and −398G>A), 7 polymorphisms at coding region (+15G>A, +36G>A, +243G>A, insC506, 531G>C, delA615 and 685G>A), the +644G>T polymorphism, and 1 haplotype (TTGTTCCIGAC) showed different frequency distributions between HCV patients and uninfected controls. Conclusion: The results from our study suggest a possible involvement of HLA-G in the risk modulation toward HCV infection.

Original languageEnglish
Pages (from-to)135-142
Number of pages8
JournalHLA
Volume89
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Virus Diseases
HLA Antigens
Hepacivirus
Base Pairing
Untranslated Regions
Th1 Cells
Genetic Association Studies
Adaptive Immunity
Innate Immunity
Haplotypes
Exons
Immune System
Lymphocytes
T-Lymphocytes
Messenger RNA
Genes

Keywords

  • hepatitis C virus
  • human leukocyte antigen-G
  • immunity
  • single nucleotide polymorphism

ASJC Scopus subject areas

  • Genetics
  • Immunology
  • Immunology and Allergy

Cite this

HLA-G regulatory polymorphisms are associated with susceptibility to HCV infection. / Catamo, E.; Zupin, L.; Freato, Nadia; Polesello, V.; Celsi, F.; Crocè, S. L.; Masutti, Flora; Pozzato, Gabriele; Segat, L.; Crovella, S.

In: HLA, Vol. 89, No. 3, 01.03.2017, p. 135-142.

Research output: Contribution to journalArticle

Catamo, E. ; Zupin, L. ; Freato, Nadia ; Polesello, V. ; Celsi, F. ; Crocè, S. L. ; Masutti, Flora ; Pozzato, Gabriele ; Segat, L. ; Crovella, S. / HLA-G regulatory polymorphisms are associated with susceptibility to HCV infection. In: HLA. 2017 ; Vol. 89, No. 3. pp. 135-142.
@article{e77ffe3ab1794a34bce9a08987f9d795,
title = "HLA-G regulatory polymorphisms are associated with susceptibility to HCV infection",
abstract = "Background: Hepatitis C virus (HCV) is able to bypass the immune system modulating innate and adaptive immune response and blocking T helper 1 (Th1) cell production. Because the human leukocyte antigen (HLA)-G molecule has immunomodulatory properties inhibiting the function and production of natural killer and cytotoxic lymphocyte T cells, as well as promoting shift from Th1 toward Th2 response, we hypothesized its involvement in susceptibility to HCV infection. Materials and Methods: Considering that HLA-G mRNA expression has been reported to be under genetic control, an association study was conducted analyzing 800 base pairs upstream the ATG at the 5′upstream regulator region (URR) and 850 base pairs from ATG to exon 3 and the 3′untranslated region (UTR) of HLA-G gene in Italian HCV-positive patients and uninfected controls. Results: Four 5′URR polymorphisms (−725C>G>T, −509C>G, −400G>A and −398G>A), 7 polymorphisms at coding region (+15G>A, +36G>A, +243G>A, insC506, 531G>C, delA615 and 685G>A), the +644G>T polymorphism, and 1 haplotype (TTGTTCCIGAC) showed different frequency distributions between HCV patients and uninfected controls. Conclusion: The results from our study suggest a possible involvement of HLA-G in the risk modulation toward HCV infection.",
keywords = "hepatitis C virus, human leukocyte antigen-G, immunity, single nucleotide polymorphism",
author = "E. Catamo and L. Zupin and Nadia Freato and V. Polesello and F. Celsi and Croc{\`e}, {S. L.} and Flora Masutti and Gabriele Pozzato and L. Segat and S. Crovella",
year = "2017",
month = "3",
day = "1",
doi = "10.1111/tan.12959",
language = "English",
volume = "89",
pages = "135--142",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - HLA-G regulatory polymorphisms are associated with susceptibility to HCV infection

AU - Catamo, E.

AU - Zupin, L.

AU - Freato, Nadia

AU - Polesello, V.

AU - Celsi, F.

AU - Crocè, S. L.

AU - Masutti, Flora

AU - Pozzato, Gabriele

AU - Segat, L.

AU - Crovella, S.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Background: Hepatitis C virus (HCV) is able to bypass the immune system modulating innate and adaptive immune response and blocking T helper 1 (Th1) cell production. Because the human leukocyte antigen (HLA)-G molecule has immunomodulatory properties inhibiting the function and production of natural killer and cytotoxic lymphocyte T cells, as well as promoting shift from Th1 toward Th2 response, we hypothesized its involvement in susceptibility to HCV infection. Materials and Methods: Considering that HLA-G mRNA expression has been reported to be under genetic control, an association study was conducted analyzing 800 base pairs upstream the ATG at the 5′upstream regulator region (URR) and 850 base pairs from ATG to exon 3 and the 3′untranslated region (UTR) of HLA-G gene in Italian HCV-positive patients and uninfected controls. Results: Four 5′URR polymorphisms (−725C>G>T, −509C>G, −400G>A and −398G>A), 7 polymorphisms at coding region (+15G>A, +36G>A, +243G>A, insC506, 531G>C, delA615 and 685G>A), the +644G>T polymorphism, and 1 haplotype (TTGTTCCIGAC) showed different frequency distributions between HCV patients and uninfected controls. Conclusion: The results from our study suggest a possible involvement of HLA-G in the risk modulation toward HCV infection.

AB - Background: Hepatitis C virus (HCV) is able to bypass the immune system modulating innate and adaptive immune response and blocking T helper 1 (Th1) cell production. Because the human leukocyte antigen (HLA)-G molecule has immunomodulatory properties inhibiting the function and production of natural killer and cytotoxic lymphocyte T cells, as well as promoting shift from Th1 toward Th2 response, we hypothesized its involvement in susceptibility to HCV infection. Materials and Methods: Considering that HLA-G mRNA expression has been reported to be under genetic control, an association study was conducted analyzing 800 base pairs upstream the ATG at the 5′upstream regulator region (URR) and 850 base pairs from ATG to exon 3 and the 3′untranslated region (UTR) of HLA-G gene in Italian HCV-positive patients and uninfected controls. Results: Four 5′URR polymorphisms (−725C>G>T, −509C>G, −400G>A and −398G>A), 7 polymorphisms at coding region (+15G>A, +36G>A, +243G>A, insC506, 531G>C, delA615 and 685G>A), the +644G>T polymorphism, and 1 haplotype (TTGTTCCIGAC) showed different frequency distributions between HCV patients and uninfected controls. Conclusion: The results from our study suggest a possible involvement of HLA-G in the risk modulation toward HCV infection.

KW - hepatitis C virus

KW - human leukocyte antigen-G

KW - immunity

KW - single nucleotide polymorphism

UR - http://www.scopus.com/inward/record.url?scp=85013036470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85013036470&partnerID=8YFLogxK

U2 - 10.1111/tan.12959

DO - 10.1111/tan.12959

M3 - Article

AN - SCOPUS:85013036470

VL - 89

SP - 135

EP - 142

JO - HLA

JF - HLA

SN - 2059-2302

IS - 3

ER -