HLA-unrestricted killing of HSV-1-infected mononuclear cells: Involvement of either γ/δ+ or α/β+ human cytotoxic T lymphocytes

R. Maccario, M. G. Revello, P. Comoli, D. Montagna, F. Locatelli, G. Gerna

Research output: Contribution to journalArticlepeer-review

Abstract

The characterization of HSV-specific human CTL, obtained in short term cultures by stimulating PBMC of healthy HSV-immune donors with autologous, PHA-activated, HSV-1-infected mononuclear cells, is described. CTL induced by using this technique are able to mediate a strong lytic activity against both HSV-1- and HSV-2-infected targets, whereas they do not kill autologous EBV- lymphoblastoid cell lines unless they are superinfected with HSV-1. TCR- γ/δ+ cells are mainly responsible for HSV-specific cytotoxic activity in some donors, whereas TCR-α/β+ CTL are primarily involved in other subjects. The large majority of HSV-specific CTL bearing either TCR-γ/δ or TCR-α/β also express CD8 and/or CD56 molecules. Virus-specific CTL, here described, require the expression of HLA class I Ag on the surface of target cells to mediate lytic activity. Nevertheless, the response is apparently HLA-unrestricted in that HSV-1-induced CTL are also able to lyse target cells mismatched for A, B, C, DR, and DQ loci. Our data suggest that both TCR- γ/δ+ and TCR-α/β+ CTL may play a role in the immune response to HSV in humans.

Original languageEnglish
Pages (from-to)1437-1445
Number of pages9
JournalJournal of Immunology
Volume150
Issue number4
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Immunology

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