HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation

Vincenzo Zappavigna, Luca Falciola, Manuela Helmer Citterich, Fulvio Mavilio, Marco E. Bianchi

Research output: Contribution to journalArticle

207 Citations (Scopus)

Abstract

High mobility group protein 1 (HMG1) is a nonhistone, chromatin-associated nuclear protein with a proposed role in the regulation of eukaryotic gene expression. We show that HMG1 interacts with proteins encoded by the HOX gene family by establishing protein-protein contacts between the HMG box domains and the HOX homeodomain. The functional role of these interactions was studied using the transcriptional activity of the human HOXD9 protein as a model, HMG1 enhances, in a dose-dependent fashion, the sequence-specific DNA binding activity in vitro, and the transcriptional activation in a co-transfection assay in vivo, of the HOXD9 protein. Functional interaction between HMG1 and HOXD9 is dependent on the DNA binding activity of the homeodomain, and requires the HOXD9 transcriptional activation domain, HMG1 enhances activation by HOXD9, but not by HOXD8, of the HOXD9-controlled element. Specific target recognition and functional interaction with HMG1 can be transferred to HOXD8 by homeodomain swapping. We propose that HMG1-like proteins might be general co-factors in HOX-mediated transcriptional activation, which facilitate access of HOX proteins to specific DNA targets, and/or introduce architectural constraints in the assembly of HOX-containing transcriptional complexes.

Original languageEnglish
Pages (from-to)4981-4991
Number of pages11
JournalEMBO Journal
Volume15
Issue number18
Publication statusPublished - Sep 16 1996

Fingerprint

High Mobility Group Proteins
Transcriptional Activation
Chemical activation
DNA
Proteins
HMG-Box Domains
Gene Expression Regulation
Nuclear Proteins
Gene expression
Chromatin
Transfection
Assays
Genes

Keywords

  • Chromatin
  • High mobility group
  • Homeodomain
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Zappavigna, V., Falciola, L., Citterich, M. H., Mavilio, F., & Bianchi, M. E. (1996). HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. EMBO Journal, 15(18), 4981-4991.

HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. / Zappavigna, Vincenzo; Falciola, Luca; Citterich, Manuela Helmer; Mavilio, Fulvio; Bianchi, Marco E.

In: EMBO Journal, Vol. 15, No. 18, 16.09.1996, p. 4981-4991.

Research output: Contribution to journalArticle

Zappavigna, V, Falciola, L, Citterich, MH, Mavilio, F & Bianchi, ME 1996, 'HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation', EMBO Journal, vol. 15, no. 18, pp. 4981-4991.
Zappavigna V, Falciola L, Citterich MH, Mavilio F, Bianchi ME. HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. EMBO Journal. 1996 Sep 16;15(18):4981-4991.
Zappavigna, Vincenzo ; Falciola, Luca ; Citterich, Manuela Helmer ; Mavilio, Fulvio ; Bianchi, Marco E. / HMG1 interacts with HOX proteins and enhances their DNA binding and transcriptional activation. In: EMBO Journal. 1996 ; Vol. 15, No. 18. pp. 4981-4991.
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