TY - JOUR
T1 - HMGA1 protein over-expression is a frequent feature of epithelial ovarian carcinomas
AU - Masciullo, Valeria
AU - Baldassarre, Gustavo
AU - Pentimalli, Francesca
AU - Berlingieri, Maria Teresa
AU - Boccia, Angelo
AU - Chiappetta, Gennaro
AU - Palazzo, Juan
AU - Manfioletti, Guidalberto
AU - Giancotti, Vincenzo
AU - Viglietto, Giuseppe
AU - Scambia, Giovanni
AU - Fusco, Alfredo
PY - 2003/7/1
Y1 - 2003/7/1
N2 - High mobility group A 1 (HMGA1) proteins are chromatinic factors, which are absent or expressed at very low levels in normal adult tissues, while they are overexpressed in several human malignant tumors. In this study, HMGA1 protein expression was investigated by immunohistochemistry in a series of 44 epithelial ovarian specimens, which included four normal ovarian tissues, 29 primary invasive carcinomas, one metastatic ovarian tumor and 10 low malignant potential (LMP) tumors. HMGA1 staining was not detected in normal ovarian surface epithelium, which is the area from which ovarian adenocarcinoma frequently arises. HMGA1 proteins were expressed at low levels in some LMP tumors, whereas they were present in abundance in most of the primary ovarian adenocarcinomas. RT-PCR and western blot analysis correlated with immunohistochemical data. We demonstrated that the suppression of HMGA1 protein synthesis by an adenovirus carrying the HMGA1 gene in antisense orientation (Ad-Yas-GFP) inhibited the growth of two human ovarian carcinoma cell lines (OVCAR-5 and OVCAR-8). These results confirm HMGA1 over-expression as a general feature of human malignant neoplasias, including ovarian cancer and suggest that suppression of HMGA1 protein synthesis by an antisense adenoviral vector may represent a new and promising gene therapy for the treatment of ovarian cancer.
AB - High mobility group A 1 (HMGA1) proteins are chromatinic factors, which are absent or expressed at very low levels in normal adult tissues, while they are overexpressed in several human malignant tumors. In this study, HMGA1 protein expression was investigated by immunohistochemistry in a series of 44 epithelial ovarian specimens, which included four normal ovarian tissues, 29 primary invasive carcinomas, one metastatic ovarian tumor and 10 low malignant potential (LMP) tumors. HMGA1 staining was not detected in normal ovarian surface epithelium, which is the area from which ovarian adenocarcinoma frequently arises. HMGA1 proteins were expressed at low levels in some LMP tumors, whereas they were present in abundance in most of the primary ovarian adenocarcinomas. RT-PCR and western blot analysis correlated with immunohistochemical data. We demonstrated that the suppression of HMGA1 protein synthesis by an adenovirus carrying the HMGA1 gene in antisense orientation (Ad-Yas-GFP) inhibited the growth of two human ovarian carcinoma cell lines (OVCAR-5 and OVCAR-8). These results confirm HMGA1 over-expression as a general feature of human malignant neoplasias, including ovarian cancer and suggest that suppression of HMGA1 protein synthesis by an antisense adenoviral vector may represent a new and promising gene therapy for the treatment of ovarian cancer.
UR - http://www.scopus.com/inward/record.url?scp=0041668179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0041668179&partnerID=8YFLogxK
U2 - 10.1093/carcin/bgg075
DO - 10.1093/carcin/bgg075
M3 - Article
C2 - 12807722
AN - SCOPUS:0041668179
VL - 24
SP - 1191
EP - 1198
JO - Carcinogenesis
JF - Carcinogenesis
SN - 0143-3334
IS - 7
ER -