HMGI(Y) and HMGI-C genes are expressed in neuroblastoma cell lines and tumors and affect retinoic acid responsiveness

Giuseppe Giannini, Lucia Di Marcotullio, Elisabetta Ristori, Massimo Zani, Marco Crescenzi, Susanna Scarpa, Giulia Piaggio, Alessandra Vacca, Fiorenzo A. Peverali, Francesca Diana, Isabella Screpanti, Luigi Frati, Alberto Gulino

Research output: Contribution to journalArticle

Abstract

HMGI-C and HMGI(Y) are architectural DNA-binding proteins that participate in the conformational regulation of active chromatin. Their pattern of expression in embryonal and adult tissues, the analysis of the 'pygmy' phenotype induced by the inactivation of the HMGI-C gene, and their frequent qualitative or quantitative alteration in experimental and human tumors indicate their pivotal role in the control of cell growth, differentiation, and tumorigenesis in several tissues representative of the epithelial, mesenchymal, and hematopoietic lineages. In contrast, very little information is available on their expression and function in neural cells. Here, we investigated the expression of the HMGI(Y) and HMGI-C genes in neuroblastoma (NB), a tumor arising from an alteration of the normal differentiation of neural crest-derived cells and in embryonal and adult adrenal tissue. Although HMGI(Y) is constitutively expressed in the embryonal and adult adrenal gland and in all of the NB cell lines and ex vivo tumors examined, its regulation appears to be associated to growth inhibition and differentiation because we observed that HMGI(Y) expression is reduced by retinoic acid (RA) in several NB cell lines that are induced to differentiate into postmitotic neurons, whereas it is up-regulated by RA in cells that fail to differentiate. Furthermore, the decrease of HMGI(Y) expression observed in RA-induced growth arrest and differentiation is abrogated in cells that have been made insensitive to this drug by NMYC overexpression. In contrast, HMGI- C expression is down-regulated during the development of the adrenal gland, completely absent in the adult individual, and only detectable in a subset of ex vivo NB tumors and in RA-resistant NB cell lines. We provide evidence of a causal link between HMGI-C expression and resistance to the growth arrest induced by RA in NB cell lines because exogenous HMGI-C expression in HMGI- C-negative and RA-sensitive cells is sufficient to convert them into RA- resistant cells. Therefore, we suggest that HMGI-C and HMGI(Y) may participate in growth- and differentiation-related tumor progression events of neuroectodermal derivatives.

Original languageEnglish
Pages (from-to)2484-2492
Number of pages9
JournalCancer Research
Volume59
Issue number10
Publication statusPublished - May 15 1999

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Giannini, G., Di Marcotullio, L., Ristori, E., Zani, M., Crescenzi, M., Scarpa, S., Piaggio, G., Vacca, A., Peverali, F. A., Diana, F., Screpanti, I., Frati, L., & Gulino, A. (1999). HMGI(Y) and HMGI-C genes are expressed in neuroblastoma cell lines and tumors and affect retinoic acid responsiveness. Cancer Research, 59(10), 2484-2492.