Abstract
Background and Objectives. Significant progress has been achieved during the past 10 years in cell transplantation and recent research has focused on the possibility of improving ventricular function after myocardial infarction. Most studies in the field of cardiac tissue repair are performed by direct intramyocardial injection of cells of different origin. Since this approach requires a surgical intervention, in this study we investigated the feasibility of non-invasive administration of bone marrow mononuclear cells (BMMNCs) by assessing the fate of peripherally injected, purified, labeled cells in cryodamaged hearts. Design and Methods. Ten donor and ten recipient inbred isogenic adult (4 weeks old) Fisher rats were used as models to mimic autologous transplantation. Myocardial damage was obtained in recipient rats by placing a frozen metal probe on the anterior left ventricular wall for 15 seconds (freeze-thaw injury technique). BMMNCs were purified and labeled with a red fluorescent cell dye. Seven days after the injury about 15-25×106 cells were infused through the femoral vein of recipient rats. Seven days after the infusion, the heart, lungs, liver, kidneys, spleen and thymus were harvested to track transplanted cells. Results. Labeled cells were found only in the injured area of the heart and not in the normal tissue; a limited number of cells were also identified in the spleen of all the animals. Most of the labeled cells in the infarcted area were Thy-1+ and some were CD34+. Interpretation and Conclusions. Our data suggest that peripherally injected BMMNCs can traffic through the circulation to the site of damage; we hypothesize that tissue injury leads to the priming of a cytokine cascade acting as chemoattractant for the infused cells.
| Original language | English |
|---|---|
| Pages (from-to) | 614-621 |
| Number of pages | 8 |
| Journal | Haematologica |
| Volume | 88 |
| Issue number | 6 |
| Publication status | Published - Jun 1 2003 |
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Keywords
- Bone marrow
- Cryoinduced myocardial damage
- Systemic injection
ASJC Scopus subject areas
- Hematology
Cite this
Homing of peripherally injected bone marrow cells in the rat after experimental myocardial injury. / Ciulla, Michele M.; Lazzari, Lorenza; Pacchiana, Raffaella; Esposito, Arturo; Bosari, Silvano; Ferrero, Stefano; Gianelli, Umberto; Paliotti, Roberta; Busca, Giuseppe; Giorgetti, Alessandra; Magrini, Fabio; Rebulla, Paolo.
In: Haematologica, Vol. 88, No. 6, 01.06.2003, p. 614-621.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Homing of peripherally injected bone marrow cells in the rat after experimental myocardial injury
AU - Ciulla, Michele M.
AU - Lazzari, Lorenza
AU - Pacchiana, Raffaella
AU - Esposito, Arturo
AU - Bosari, Silvano
AU - Ferrero, Stefano
AU - Gianelli, Umberto
AU - Paliotti, Roberta
AU - Busca, Giuseppe
AU - Giorgetti, Alessandra
AU - Magrini, Fabio
AU - Rebulla, Paolo
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Background and Objectives. Significant progress has been achieved during the past 10 years in cell transplantation and recent research has focused on the possibility of improving ventricular function after myocardial infarction. Most studies in the field of cardiac tissue repair are performed by direct intramyocardial injection of cells of different origin. Since this approach requires a surgical intervention, in this study we investigated the feasibility of non-invasive administration of bone marrow mononuclear cells (BMMNCs) by assessing the fate of peripherally injected, purified, labeled cells in cryodamaged hearts. Design and Methods. Ten donor and ten recipient inbred isogenic adult (4 weeks old) Fisher rats were used as models to mimic autologous transplantation. Myocardial damage was obtained in recipient rats by placing a frozen metal probe on the anterior left ventricular wall for 15 seconds (freeze-thaw injury technique). BMMNCs were purified and labeled with a red fluorescent cell dye. Seven days after the injury about 15-25×106 cells were infused through the femoral vein of recipient rats. Seven days after the infusion, the heart, lungs, liver, kidneys, spleen and thymus were harvested to track transplanted cells. Results. Labeled cells were found only in the injured area of the heart and not in the normal tissue; a limited number of cells were also identified in the spleen of all the animals. Most of the labeled cells in the infarcted area were Thy-1+ and some were CD34+. Interpretation and Conclusions. Our data suggest that peripherally injected BMMNCs can traffic through the circulation to the site of damage; we hypothesize that tissue injury leads to the priming of a cytokine cascade acting as chemoattractant for the infused cells.
AB - Background and Objectives. Significant progress has been achieved during the past 10 years in cell transplantation and recent research has focused on the possibility of improving ventricular function after myocardial infarction. Most studies in the field of cardiac tissue repair are performed by direct intramyocardial injection of cells of different origin. Since this approach requires a surgical intervention, in this study we investigated the feasibility of non-invasive administration of bone marrow mononuclear cells (BMMNCs) by assessing the fate of peripherally injected, purified, labeled cells in cryodamaged hearts. Design and Methods. Ten donor and ten recipient inbred isogenic adult (4 weeks old) Fisher rats were used as models to mimic autologous transplantation. Myocardial damage was obtained in recipient rats by placing a frozen metal probe on the anterior left ventricular wall for 15 seconds (freeze-thaw injury technique). BMMNCs were purified and labeled with a red fluorescent cell dye. Seven days after the injury about 15-25×106 cells were infused through the femoral vein of recipient rats. Seven days after the infusion, the heart, lungs, liver, kidneys, spleen and thymus were harvested to track transplanted cells. Results. Labeled cells were found only in the injured area of the heart and not in the normal tissue; a limited number of cells were also identified in the spleen of all the animals. Most of the labeled cells in the infarcted area were Thy-1+ and some were CD34+. Interpretation and Conclusions. Our data suggest that peripherally injected BMMNCs can traffic through the circulation to the site of damage; we hypothesize that tissue injury leads to the priming of a cytokine cascade acting as chemoattractant for the infused cells.
KW - Bone marrow
KW - Cryoinduced myocardial damage
KW - Systemic injection
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M3 - Article
C2 - 12801836
AN - SCOPUS:10744222477
VL - 88
SP - 614
EP - 621
JO - Haematologica
JF - Haematologica
SN - 0390-6078
IS - 6
ER -