Homocysteine, cysteine, folate and vitamin B12 status in type 2 diabetic patients with chronic kidney disease

Anna Pastore, Annalisa Noce, Gianna Di Giovamberardino, Alessandro De Stefano, Cinzia Callà, Rossella Zenobi, Mariarita Dessì, Nicola Di Daniele

Research output: Contribution to journalArticlepeer-review


Background: Hyperhomocysteinemia (hHcy) is a risk factor in the progression of chronic kidney disease (CKD). In type 2 diabetes (T2D), hHcy is strongly associated with increased risk of cardiovascular disease. Vitamin B12 and folic acid supplementation have been reported to lower homocysteine (tHcy) levels, but no data on plasma tHcy, cysteine (Cys), folate and vitamin B12 levels in T2D-CKD patients are reported. Procedures: tHcy and Cys levels were analyzed in 178 T2D-CKD patients by high performance liquid chromatography (HPLC) with fluorescence detection. In addition, we determined folate and vitamin B12 levels using a chemiluminescence method. Results: tHcy and Cys levels were increased in T2D patients, and this rise positively correlated with the CKD stage (P 12 levels were lower, except at stage IV. We did not find any correlation between B-vitamins and levels of tHcy and Cys, regardless of the CKD stage. Conclusions: This is the first study reporting tHcy, Cys and B-vitamins status in T2D-CKD patients. Although limited to our cohort of 178 patients, our findings could be helpful in clarifying the conflicting literature regarding B-vitamins supplementation. Further studies are necessary before any Hcy-lowering therapy can be safely established in T2D-CKD subjects.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalJournal of Nephrology
Issue number5
Publication statusPublished - Oct 13 2015


  • Chronic kidney disease
  • Cysteine
  • Folate
  • Homocysteine
  • Type 2 diabetes
  • Vitamin B12

ASJC Scopus subject areas

  • Nephrology


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