TY - JOUR
T1 - Homocysteine, cysteine, folate and vitamin B12 status in type 2 diabetic patients with chronic kidney disease
AU - Pastore, Anna
AU - Noce, Annalisa
AU - Di Giovamberardino, Gianna
AU - De Stefano, Alessandro
AU - Callà, Cinzia
AU - Zenobi, Rossella
AU - Dessì, Mariarita
AU - Di Daniele, Nicola
PY - 2015/10/13
Y1 - 2015/10/13
N2 - Background: Hyperhomocysteinemia (hHcy) is a risk factor in the progression of chronic kidney disease (CKD). In type 2 diabetes (T2D), hHcy is strongly associated with increased risk of cardiovascular disease. Vitamin B12 and folic acid supplementation have been reported to lower homocysteine (tHcy) levels, but no data on plasma tHcy, cysteine (Cys), folate and vitamin B12 levels in T2D-CKD patients are reported. Procedures: tHcy and Cys levels were analyzed in 178 T2D-CKD patients by high performance liquid chromatography (HPLC) with fluorescence detection. In addition, we determined folate and vitamin B12 levels using a chemiluminescence method. Results: tHcy and Cys levels were increased in T2D patients, and this rise positively correlated with the CKD stage (P 12 levels were lower, except at stage IV. We did not find any correlation between B-vitamins and levels of tHcy and Cys, regardless of the CKD stage. Conclusions: This is the first study reporting tHcy, Cys and B-vitamins status in T2D-CKD patients. Although limited to our cohort of 178 patients, our findings could be helpful in clarifying the conflicting literature regarding B-vitamins supplementation. Further studies are necessary before any Hcy-lowering therapy can be safely established in T2D-CKD subjects.
AB - Background: Hyperhomocysteinemia (hHcy) is a risk factor in the progression of chronic kidney disease (CKD). In type 2 diabetes (T2D), hHcy is strongly associated with increased risk of cardiovascular disease. Vitamin B12 and folic acid supplementation have been reported to lower homocysteine (tHcy) levels, but no data on plasma tHcy, cysteine (Cys), folate and vitamin B12 levels in T2D-CKD patients are reported. Procedures: tHcy and Cys levels were analyzed in 178 T2D-CKD patients by high performance liquid chromatography (HPLC) with fluorescence detection. In addition, we determined folate and vitamin B12 levels using a chemiluminescence method. Results: tHcy and Cys levels were increased in T2D patients, and this rise positively correlated with the CKD stage (P 12 levels were lower, except at stage IV. We did not find any correlation between B-vitamins and levels of tHcy and Cys, regardless of the CKD stage. Conclusions: This is the first study reporting tHcy, Cys and B-vitamins status in T2D-CKD patients. Although limited to our cohort of 178 patients, our findings could be helpful in clarifying the conflicting literature regarding B-vitamins supplementation. Further studies are necessary before any Hcy-lowering therapy can be safely established in T2D-CKD subjects.
KW - Chronic kidney disease
KW - Cysteine
KW - Folate
KW - Homocysteine
KW - Type 2 diabetes
KW - Vitamin B12
UR - http://www.scopus.com/inward/record.url?scp=84941219387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941219387&partnerID=8YFLogxK
U2 - 10.1007/s40620-014-0126-4
DO - 10.1007/s40620-014-0126-4
M3 - Article
C2 - 25095762
AN - SCOPUS:84941219387
VL - 28
SP - 571
EP - 576
JO - Journal of Nephrology
JF - Journal of Nephrology
SN - 1121-8428
IS - 5
ER -