Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets

Rafael Fernandes Zanin, Luís Felipe Ingrassia Campesato, Elizandra Braganhol, Maria Rosa Chitolina Schetinger, Angela Terezinha de Souza Wyse, Ana Maria Oliveira Battastini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hyperhomocysteinemia is an independent risk factor for atherothrombotic disease. Platelets play an important role in cardiovascular disease and release pro-aggregates mediators when activated, such as ADP, a physiological agonist involved in normal hemostasis and thrombosis. NTPDases and 5'-nucleotidase are ecto-enzymes that hydrolyze ATP, ADP and AMP to adenosine playing an important role on blood flow and thrombogenesis by regulating ADP catabolism. The aim of the present study was evaluate extracellular adenine nucleotide hydrolysis of rat platelets exposed to homocysteine in vitro and in vivo. In vitro homocysteine (Hcy) in the concentration range of 20 to 500 μM caused a significant decrease on ATP (around 30%) and ADP (around 45%) hydrolysis, respectively, while AMP hydrolysis was not altered. Hcy was not able to inhibit the hydrolysis of ATP and ADP catalyzed by purified apyrase at the same concentrations tested in vitro on platelets, suggesting an indirect effect. The inhibitory effect of Hcy on platelets was prevented by antioxidants agents in vitro and in vivo. Furthermore homocysteine treatment increased platelet aggregation induced by ADP. Based on the results presented herein, we propose that inhibition of extracellular ATP and ADP hydrolysis caused by homocysteine was probably due oxidative stress, since antioxidants prevented such effects. These findings may contribute to an increase platelet response to ADP and consequence development of thrombotic risk attributed to hyperhomocysteinemia.

Original languageEnglish
JournalThrombosis Research
Volume125
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Homocysteine
Adenosine Diphosphate
Hydrolysis
Blood Platelets
Nucleotides
Adenosine Triphosphate
Hyperhomocysteinemia
Adenosine Monophosphate
Antioxidants
Apyrase
5'-Nucleotidase
Adenine Nucleotides
Hemostasis
Platelet Aggregation
Adenosine
Oxidative Stress
Thrombosis
Cardiovascular Diseases
In Vitro Techniques
Enzymes

Keywords

  • ADP
  • ATP
  • Homocysteine
  • NTPDase
  • Platelet

ASJC Scopus subject areas

  • Medicine(all)
  • Hematology

Cite this

Fernandes Zanin, R., Ingrassia Campesato, L. F., Braganhol, E., Chitolina Schetinger, M. R., de Souza Wyse, A. T., & Oliveira Battastini, A. M. (2010). Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets. Thrombosis Research, 125(3). https://doi.org/10.1016/j.thromres.2009.09.020

Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets. / Fernandes Zanin, Rafael; Ingrassia Campesato, Luís Felipe; Braganhol, Elizandra; Chitolina Schetinger, Maria Rosa; de Souza Wyse, Angela Terezinha; Oliveira Battastini, Ana Maria.

In: Thrombosis Research, Vol. 125, No. 3, 03.2010.

Research output: Contribution to journalArticle

Fernandes Zanin, R, Ingrassia Campesato, LF, Braganhol, E, Chitolina Schetinger, MR, de Souza Wyse, AT & Oliveira Battastini, AM 2010, 'Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets', Thrombosis Research, vol. 125, no. 3. https://doi.org/10.1016/j.thromres.2009.09.020
Fernandes Zanin R, Ingrassia Campesato LF, Braganhol E, Chitolina Schetinger MR, de Souza Wyse AT, Oliveira Battastini AM. Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets. Thrombosis Research. 2010 Mar;125(3). https://doi.org/10.1016/j.thromres.2009.09.020
Fernandes Zanin, Rafael ; Ingrassia Campesato, Luís Felipe ; Braganhol, Elizandra ; Chitolina Schetinger, Maria Rosa ; de Souza Wyse, Angela Terezinha ; Oliveira Battastini, Ana Maria. / Homocysteine decreases extracellular nucleotide hydrolysis in rat platelets. In: Thrombosis Research. 2010 ; Vol. 125, No. 3.
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