Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome

Francesco Orio, Stefano Palomba, Sebastiano Di Biase, Annamaria Colao, Libuse Tauchmanova, Silvia Savastano, Donato Labella, Tiziana Russo, Fulvio Zullo, Gaetano Lombardi

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hcy metabolism in young women with polycystic ovary syndrome (PCOS). Seventy young women with PCOS and another 70 healthy women with low folate intake were enrolled. Cases and controls were matched for age, body mass index, and allele frequency. Hcy, vitamin B12, and folate levels were measured, and a genetic analysis of 5,10-MTHFR at nucleotide 677 was performed in all subjects. No difference in mean Hcy levels was observed between PCOS women in comparison to the control group. Considering the different MTHFR polymorphism, no significant difference was found in serum Hcy levels between subjects with PCOS and controls showing CC (10.4 ± 3.1 vs. 9.7 ± 2.9 μmol/liter ± SD) and CT genotypes (10.9 ± 3.8 vs. 11.0 ± 3.2 μmol/liter ± SD). In subjects with a TT homozygous state, a significant (P <0.05) difference was observed between PCOS and control women (11.5 ± 3.9 vs. 22.0 ± 7.8 μmol/liter ± SD). In conclusion, our data show that in PCOS women, the serum Hcy levels are normal, and the C677T polymorphism of MTHFR does not influence the Hcy levels like in controls.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

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    Orio, F., Palomba, S., Di Biase, S., Colao, A., Tauchmanova, L., Savastano, S., Labella, D., Russo, T., Zullo, F., & Lombardi, G. (2003). Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome. Journal of Clinical Endocrinology and Metabolism, 88(2), 673-679. https://doi.org/10.1210/jc.2002-021142