Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome

Francesco Orio, Stefano Palomba, Sebastiano Di Biase, Annamaria Colao, Libuse Tauchmanova, Silvia Savastano, Donato Labella, Tiziana Russo, Fulvio Zullo, Gaetano Lombardi

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The aim of this study was to investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hcy metabolism in young women with polycystic ovary syndrome (PCOS). Seventy young women with PCOS and another 70 healthy women with low folate intake were enrolled. Cases and controls were matched for age, body mass index, and allele frequency. Hcy, vitamin B12, and folate levels were measured, and a genetic analysis of 5,10-MTHFR at nucleotide 677 was performed in all subjects. No difference in mean Hcy levels was observed between PCOS women in comparison to the control group. Considering the different MTHFR polymorphism, no significant difference was found in serum Hcy levels between subjects with PCOS and controls showing CC (10.4 ± 3.1 vs. 9.7 ± 2.9 μmol/liter ± SD) and CT genotypes (10.9 ± 3.8 vs. 11.0 ± 3.2 μmol/liter ± SD). In subjects with a TT homozygous state, a significant (P <0.05) difference was observed between PCOS and control women (11.5 ± 3.9 vs. 22.0 ± 7.8 μmol/liter ± SD). In conclusion, our data show that in PCOS women, the serum Hcy levels are normal, and the C677T polymorphism of MTHFR does not influence the Hcy levels like in controls.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalJournal of Clinical Endocrinology and Metabolism
Volume88
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Methylenetetrahydrofolate Reductase (NADPH2)
Polycystic Ovary Syndrome
Homocysteine
Polymorphism
Folic Acid
Vitamin B 12
Serum
Gene Frequency
Metabolism
Body Mass Index
Nucleotides
Genotype
Control Groups

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome. / Orio, Francesco; Palomba, Stefano; Di Biase, Sebastiano; Colao, Annamaria; Tauchmanova, Libuse; Savastano, Silvia; Labella, Donato; Russo, Tiziana; Zullo, Fulvio; Lombardi, Gaetano.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 88, No. 2, 01.02.2003, p. 673-679.

Research output: Contribution to journalArticle

Orio, F, Palomba, S, Di Biase, S, Colao, A, Tauchmanova, L, Savastano, S, Labella, D, Russo, T, Zullo, F & Lombardi, G 2003, 'Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome', Journal of Clinical Endocrinology and Metabolism, vol. 88, no. 2, pp. 673-679. https://doi.org/10.1210/jc.2002-021142
Orio, Francesco ; Palomba, Stefano ; Di Biase, Sebastiano ; Colao, Annamaria ; Tauchmanova, Libuse ; Savastano, Silvia ; Labella, Donato ; Russo, Tiziana ; Zullo, Fulvio ; Lombardi, Gaetano. / Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome. In: Journal of Clinical Endocrinology and Metabolism. 2003 ; Vol. 88, No. 2. pp. 673-679.
@article{a13f62547fab478b9e88808e0fd54866,
title = "Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome",
abstract = "The aim of this study was to investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hcy metabolism in young women with polycystic ovary syndrome (PCOS). Seventy young women with PCOS and another 70 healthy women with low folate intake were enrolled. Cases and controls were matched for age, body mass index, and allele frequency. Hcy, vitamin B12, and folate levels were measured, and a genetic analysis of 5,10-MTHFR at nucleotide 677 was performed in all subjects. No difference in mean Hcy levels was observed between PCOS women in comparison to the control group. Considering the different MTHFR polymorphism, no significant difference was found in serum Hcy levels between subjects with PCOS and controls showing CC (10.4 ± 3.1 vs. 9.7 ± 2.9 μmol/liter ± SD) and CT genotypes (10.9 ± 3.8 vs. 11.0 ± 3.2 μmol/liter ± SD). In subjects with a TT homozygous state, a significant (P <0.05) difference was observed between PCOS and control women (11.5 ± 3.9 vs. 22.0 ± 7.8 μmol/liter ± SD). In conclusion, our data show that in PCOS women, the serum Hcy levels are normal, and the C677T polymorphism of MTHFR does not influence the Hcy levels like in controls.",
author = "Francesco Orio and Stefano Palomba and {Di Biase}, Sebastiano and Annamaria Colao and Libuse Tauchmanova and Silvia Savastano and Donato Labella and Tiziana Russo and Fulvio Zullo and Gaetano Lombardi",
year = "2003",
month = "2",
day = "1",
doi = "10.1210/jc.2002-021142",
language = "English",
volume = "88",
pages = "673--679",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "2",

}

TY - JOUR

T1 - Homocysteine levels and C677T polymorphism of methylenetetrahydrofolate reductase in women with polycystic ovary syndrome

AU - Orio, Francesco

AU - Palomba, Stefano

AU - Di Biase, Sebastiano

AU - Colao, Annamaria

AU - Tauchmanova, Libuse

AU - Savastano, Silvia

AU - Labella, Donato

AU - Russo, Tiziana

AU - Zullo, Fulvio

AU - Lombardi, Gaetano

PY - 2003/2/1

Y1 - 2003/2/1

N2 - The aim of this study was to investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hcy metabolism in young women with polycystic ovary syndrome (PCOS). Seventy young women with PCOS and another 70 healthy women with low folate intake were enrolled. Cases and controls were matched for age, body mass index, and allele frequency. Hcy, vitamin B12, and folate levels were measured, and a genetic analysis of 5,10-MTHFR at nucleotide 677 was performed in all subjects. No difference in mean Hcy levels was observed between PCOS women in comparison to the control group. Considering the different MTHFR polymorphism, no significant difference was found in serum Hcy levels between subjects with PCOS and controls showing CC (10.4 ± 3.1 vs. 9.7 ± 2.9 μmol/liter ± SD) and CT genotypes (10.9 ± 3.8 vs. 11.0 ± 3.2 μmol/liter ± SD). In subjects with a TT homozygous state, a significant (P <0.05) difference was observed between PCOS and control women (11.5 ± 3.9 vs. 22.0 ± 7.8 μmol/liter ± SD). In conclusion, our data show that in PCOS women, the serum Hcy levels are normal, and the C677T polymorphism of MTHFR does not influence the Hcy levels like in controls.

AB - The aim of this study was to investigate the homocysteine (Hcy) levels and the C677T polymorphism of 5,10-methylenetetrahydrofolate reductase (MTHFR), a crucial factor of the Hcy metabolism in young women with polycystic ovary syndrome (PCOS). Seventy young women with PCOS and another 70 healthy women with low folate intake were enrolled. Cases and controls were matched for age, body mass index, and allele frequency. Hcy, vitamin B12, and folate levels were measured, and a genetic analysis of 5,10-MTHFR at nucleotide 677 was performed in all subjects. No difference in mean Hcy levels was observed between PCOS women in comparison to the control group. Considering the different MTHFR polymorphism, no significant difference was found in serum Hcy levels between subjects with PCOS and controls showing CC (10.4 ± 3.1 vs. 9.7 ± 2.9 μmol/liter ± SD) and CT genotypes (10.9 ± 3.8 vs. 11.0 ± 3.2 μmol/liter ± SD). In subjects with a TT homozygous state, a significant (P <0.05) difference was observed between PCOS and control women (11.5 ± 3.9 vs. 22.0 ± 7.8 μmol/liter ± SD). In conclusion, our data show that in PCOS women, the serum Hcy levels are normal, and the C677T polymorphism of MTHFR does not influence the Hcy levels like in controls.

UR - http://www.scopus.com/inward/record.url?scp=0037326227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037326227&partnerID=8YFLogxK

U2 - 10.1210/jc.2002-021142

DO - 10.1210/jc.2002-021142

M3 - Article

C2 - 12574198

AN - SCOPUS:0037326227

VL - 88

SP - 673

EP - 679

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 2

ER -