Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus

M. G. Signorello, G. L. Viviani, U. Armani, R. Cerone, G. Minniti, A. Piana, G. Leoncini

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Introduction: Type 2 diabetes mellitus shows a characteristic altered platelet function that can be due to several mechanisms such as oxidative stress. Hyperhomocysteinemia, considered as a risk factor for various arterial thrombosis, may have a role in generating oxidative damage, even if the pathogenic mechanisms are still not clear. In this report we aimed to determine the role of plasma homocysteine in inducing oxidative stress in type 2 diabetes mellitus. Materials and methods: The study was performed on a group of 34 males with type 2 diabetes and 36 healthy subjects matched for sex and age. Patients and healthy subjects were undergone to laboratory evaluation for plasma homocysteine levels and other metabolic parameters. In both groups of subjects platelet reactive oxygen species, nitric oxide and guanosine 3′,5′ cyclic monophosphate levels were measured. Moreover the reduced glutathione content in platelets of patients and of healthy subjects was assayed. Results: Plasma homocysteine levels were significantly increased in patients compared with healthy subjects. The basal level of reactive oxygen species was significantly higher in patients than in controls. In addition platelets of patients stimulated with thrombin produced more reactive oxygen species than healthy subjects ones. The nitric oxide, guanosine 3′,5′ cyclic monophosphate and reduced glutathione content were decreased in platelets of patients. Conclusions: As homocysteine stimulates oxidative stress and inhibits nitric oxide formation, hyperhomocysteinemia measured in type 2 diabetic patients, promoting platelet hyperactivity, could have a role in the atherogenic effects described in type 2 diabetes.

Original languageEnglish
Pages (from-to)607-613
Number of pages7
JournalThrombosis Research
Volume120
Issue number4
DOIs
Publication statusPublished - 2007

Fingerprint

Homocysteine
Type 2 Diabetes Mellitus
Reactive Oxygen Species
Nitric Oxide
Blood Platelets
Healthy Volunteers
Hyperhomocysteinemia
Oxidative Stress
Guanosine
Glutathione
Thrombin
Thrombosis

Keywords

  • Diabetes
  • GSH
  • Homocysteine
  • Human platelets
  • Nitric oxide
  • Oxidative stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Signorello, M. G., Viviani, G. L., Armani, U., Cerone, R., Minniti, G., Piana, A., & Leoncini, G. (2007). Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus. Thrombosis Research, 120(4), 607-613. https://doi.org/10.1016/j.thromres.2006.11.008

Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus. / Signorello, M. G.; Viviani, G. L.; Armani, U.; Cerone, R.; Minniti, G.; Piana, A.; Leoncini, G.

In: Thrombosis Research, Vol. 120, No. 4, 2007, p. 607-613.

Research output: Contribution to journalArticle

Signorello, MG, Viviani, GL, Armani, U, Cerone, R, Minniti, G, Piana, A & Leoncini, G 2007, 'Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus', Thrombosis Research, vol. 120, no. 4, pp. 607-613. https://doi.org/10.1016/j.thromres.2006.11.008
Signorello MG, Viviani GL, Armani U, Cerone R, Minniti G, Piana A et al. Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus. Thrombosis Research. 2007;120(4):607-613. https://doi.org/10.1016/j.thromres.2006.11.008
Signorello, M. G. ; Viviani, G. L. ; Armani, U. ; Cerone, R. ; Minniti, G. ; Piana, A. ; Leoncini, G. / Homocysteine, reactive oxygen species and nitric oxide in type 2 diabetes mellitus. In: Thrombosis Research. 2007 ; Vol. 120, No. 4. pp. 607-613.
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