Homologous prime boosting based on intranasal delivery of non-pathogenic invasive Escherichia coli expressing MPT64, decreases Mycobacterium tuberculosis dissemination

Michela Sali, Elisa Dainese, Matteo Morandi, Antonella Zumbo, Stefano Rocca, Sylvie Goussard, Giorgio Palù, Catherine Grillot-Courvalin, Giovanni Delogu, Riccardo Manganelli

Research output: Contribution to journalArticlepeer-review

Abstract

Protein-subunit vaccines as boosting strategies against tuberculosis (TB) infection are currently in the pipeline of TB vaccine research. Their main limitation is represented by their poor immunogenicity, which makes it necessary to couple protein-subunits with adjuvant molecules. In this study, we employed replication-deficient invasive Escherichia coli strains to deliver Mycobacterium tuberculosis proteins to the cytoplasm of non-phagocytic eukaryotic cells using various priming and prime-boosting vaccination protocols. Our results demonstrate that intranasal administration of invasive E. coli expressing the M. tuberculosis protective antigen MPT64 to mice primed with a recombinant BCG strain over-expressing MPT64 on its surface, decrease bacterial burden in mice spleens. Our data suggest that replication-deficient invasive E. coli may represent a suitable platform for BCG/rBCG priming followed by homologous-boosting immunization strategies.

Original languageEnglish
Pages (from-to)4051-4058
Number of pages8
JournalVaccine
Volume32
Issue number32
DOIs
Publication statusPublished - Jul 7 2014

Keywords

  • Attenuated vaccines: Mycobacterium tuberculosis
  • Bactofection
  • Tuberculosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine
  • Medicine(all)

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