Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function

Barbara Canonico, Manila Candiracci, Barbara Citterio, Rosa Curci, Stefano Squarzoni, Annalisa Mazzoni, Stefano Papa, Elena Piatti

Research output: Contribution to journalArticle

Abstract

Aim: Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles. Materials & methods: HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy. Results: Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria. Conclusion: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

Original languageEnglish
Pages (from-to)445-456
Number of pages12
JournalFuture Microbiology
Volume9
Issue number4
DOIs
Publication statusPublished - 2014

Keywords

  • Candida albicans
  • flavonoids
  • mitochondrial activity
  • morphogenesis
  • vacuole

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Medicine(all)

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    Canonico, B., Candiracci, M., Citterio, B., Curci, R., Squarzoni, S., Mazzoni, A., Papa, S., & Piatti, E. (2014). Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function. Future Microbiology, 9(4), 445-456. https://doi.org/10.2217/fmb.14.17