Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function

Barbara Canonico, Manila Candiracci, Barbara Citterio, Rosa Curci, Stefano Squarzoni, Annalisa Mazzoni, Stefano Papa, Elena Piatti

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Aim: Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles. Materials & methods: HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy. Results: Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria. Conclusion: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

Original languageEnglish
Pages (from-to)445-456
Number of pages12
JournalFuture Microbiology
Volume9
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Honey
Candida albicans
Mitochondrial DNA
Morphogenesis
Flavonoids
Yeasts
Electron Scanning Microscopy
Cell Cycle
Flow Cytometry
Cell Cycle Resting Phase
Membranes
Hyphae
Cell Shape
G2 Phase
G1 Phase
Organelle Biogenesis
Vacuoles
Organelles
Cell Division
Virulence

Keywords

  • Candida albicans
  • flavonoids
  • mitochondrial activity
  • morphogenesis
  • vacuole

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Medicine(all)

Cite this

Canonico, B., Candiracci, M., Citterio, B., Curci, R., Squarzoni, S., Mazzoni, A., ... Piatti, E. (2014). Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function. Future Microbiology, 9(4), 445-456. https://doi.org/10.2217/fmb.14.17

Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function. / Canonico, Barbara; Candiracci, Manila; Citterio, Barbara; Curci, Rosa; Squarzoni, Stefano; Mazzoni, Annalisa; Papa, Stefano; Piatti, Elena.

In: Future Microbiology, Vol. 9, No. 4, 2014, p. 445-456.

Research output: Contribution to journalArticle

Canonico, B, Candiracci, M, Citterio, B, Curci, R, Squarzoni, S, Mazzoni, A, Papa, S & Piatti, E 2014, 'Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function', Future Microbiology, vol. 9, no. 4, pp. 445-456. https://doi.org/10.2217/fmb.14.17
Canonico, Barbara ; Candiracci, Manila ; Citterio, Barbara ; Curci, Rosa ; Squarzoni, Stefano ; Mazzoni, Annalisa ; Papa, Stefano ; Piatti, Elena. / Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function. In: Future Microbiology. 2014 ; Vol. 9, No. 4. pp. 445-456.
@article{d242bd6a76c0418a9dd82f58eb7ef641,
title = "Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function",
abstract = "Aim: Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles. Materials & methods: HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy. Results: Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria. Conclusion: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.",
keywords = "Candida albicans, flavonoids, mitochondrial activity, morphogenesis, vacuole",
author = "Barbara Canonico and Manila Candiracci and Barbara Citterio and Rosa Curci and Stefano Squarzoni and Annalisa Mazzoni and Stefano Papa and Elena Piatti",
year = "2014",
doi = "10.2217/fmb.14.17",
language = "English",
volume = "9",
pages = "445--456",
journal = "Future Microbiology",
issn = "1746-0913",
publisher = "Future Medicine Ltd.",
number = "4",

}

TY - JOUR

T1 - Honey flavonoids inhibit Candida albicans morphogenesis by affecting DNA behavior and mitochondrial function

AU - Canonico, Barbara

AU - Candiracci, Manila

AU - Citterio, Barbara

AU - Curci, Rosa

AU - Squarzoni, Stefano

AU - Mazzoni, Annalisa

AU - Papa, Stefano

AU - Piatti, Elena

PY - 2014

Y1 - 2014

N2 - Aim: Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles. Materials & methods: HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy. Results: Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria. Conclusion: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

AB - Aim: Candida albicans is a pathogenic yeast, which forms a range of polarized and expanded cell shapes. We aimed to determine the correlation between honey extract (HFE) activity and changes in C. albicans cell cycle, morphology and subcellular organelles. Materials & methods: HFE anticandidal properties were investigated using flow cytometry and scanning electron microscopy. Results: Flow cytometry and scanning electron microscopy analyses indicated that HFE may inhibit the growth of the three phenotypes displayed by C. albicans and reduce infection by affecting membrane integrity. HFE affects hyphal transition by reducing the G0/G1 phase and increasing the G2/M phase. Conversely, yeast and pseudohyphae do not appear to be affected. Modifications of vacuolization and mitochondrial activity, during yeast-hypha transition establish the involvement of vacuole and mitochondria. Conclusion: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

KW - Candida albicans

KW - flavonoids

KW - mitochondrial activity

KW - morphogenesis

KW - vacuole

UR - http://www.scopus.com/inward/record.url?scp=84900296585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900296585&partnerID=8YFLogxK

U2 - 10.2217/fmb.14.17

DO - 10.2217/fmb.14.17

M3 - Article

VL - 9

SP - 445

EP - 456

JO - Future Microbiology

JF - Future Microbiology

SN - 1746-0913

IS - 4

ER -