TY - JOUR
T1 - Horam nonam exclamavit
T2 - Sitio. The trace proteome of your daily vinegar
AU - Di Girolamo, Francesco
AU - D'Amato, Alfonsina
AU - Righetti, Pier Giorgio
PY - 2011/12/21
Y1 - 2011/12/21
N2 - The trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at pH 2.2 in presence of 0.1% trifluoroacetic acid. A total of 27 unique gene products have been identified, of which 10 specific of the database Vitis vinifera, 13 found in the general database Uniprot_viridiplantae and 4 in Swiss Prot_all entries. The most abundant species detected, on the basis of spectral counts, appears to be the whole genome shotgun sequence of line PN40024, scaffold_22 (a protein of the glycosyl hydrolase family). Curiously, up to the present, no information had been available on vinegar proteome.
AB - The trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at pH 2.2 in presence of 0.1% trifluoroacetic acid. A total of 27 unique gene products have been identified, of which 10 specific of the database Vitis vinifera, 13 found in the general database Uniprot_viridiplantae and 4 in Swiss Prot_all entries. The most abundant species detected, on the basis of spectral counts, appears to be the whole genome shotgun sequence of line PN40024, scaffold_22 (a protein of the glycosyl hydrolase family). Curiously, up to the present, no information had been available on vinegar proteome.
KW - Combinatorial peptide ligand libraries
KW - Food traceability
KW - Trace proteome
KW - White-wine vinegar
UR - http://www.scopus.com/inward/record.url?scp=82355164360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82355164360&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2011.08.004
DO - 10.1016/j.jprot.2011.08.004
M3 - Article
C2 - 21867782
AN - SCOPUS:82355164360
VL - 75
SP - 718
EP - 724
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 2
ER -