Abstract
The trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at pH 2.2 in presence of 0.1% trifluoroacetic acid. A total of 27 unique gene products have been identified, of which 10 specific of the database Vitis vinifera, 13 found in the general database Uniprot_viridiplantae and 4 in Swiss Prot_all entries. The most abundant species detected, on the basis of spectral counts, appears to be the whole genome shotgun sequence of line PN40024, scaffold_22 (a protein of the glycosyl hydrolase family). Curiously, up to the present, no information had been available on vinegar proteome.
| Original language | English |
|---|---|
| Pages (from-to) | 718-724 |
| Number of pages | 7 |
| Journal | Journal of Proteomics |
| Volume | 75 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Dec 21 2011 |
Fingerprint
Keywords
- Combinatorial peptide ligand libraries
- Food traceability
- Trace proteome
- White-wine vinegar
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
Horam nonam exclamavit : Sitio. The trace proteome of your daily vinegar. / Di Girolamo, Francesco; D'Amato, Alfonsina; Righetti, Pier Giorgio.
In: Journal of Proteomics, Vol. 75, No. 2, 21.12.2011, p. 718-724.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Horam nonam exclamavit
T2 - Sitio. The trace proteome of your daily vinegar
AU - Di Girolamo, Francesco
AU - D'Amato, Alfonsina
AU - Righetti, Pier Giorgio
PY - 2011/12/21
Y1 - 2011/12/21
N2 - The trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at pH 2.2 in presence of 0.1% trifluoroacetic acid. A total of 27 unique gene products have been identified, of which 10 specific of the database Vitis vinifera, 13 found in the general database Uniprot_viridiplantae and 4 in Swiss Prot_all entries. The most abundant species detected, on the basis of spectral counts, appears to be the whole genome shotgun sequence of line PN40024, scaffold_22 (a protein of the glycosyl hydrolase family). Curiously, up to the present, no information had been available on vinegar proteome.
AB - The trace proteome of white-wine vinegar has been identified via capture with home-made combinatorial peptide ligand libraries under conditions mimicking reverse-phase capture, i.e. at pH 2.2 in presence of 0.1% trifluoroacetic acid. A total of 27 unique gene products have been identified, of which 10 specific of the database Vitis vinifera, 13 found in the general database Uniprot_viridiplantae and 4 in Swiss Prot_all entries. The most abundant species detected, on the basis of spectral counts, appears to be the whole genome shotgun sequence of line PN40024, scaffold_22 (a protein of the glycosyl hydrolase family). Curiously, up to the present, no information had been available on vinegar proteome.
KW - Combinatorial peptide ligand libraries
KW - Food traceability
KW - Trace proteome
KW - White-wine vinegar
UR - http://www.scopus.com/inward/record.url?scp=82355164360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=82355164360&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2011.08.004
DO - 10.1016/j.jprot.2011.08.004
M3 - Article
VL - 75
SP - 718
EP - 724
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 2
ER -