Hospital-acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014-2015

Diletta Valentini, Giovanni Ianiro, Ilaria Di Bartolo, Chiara Di Camillo, Elena Boccuzzi, Anna C. Vittucci, Franco M. Ruggeri, Marina Monini

Research output: Contribution to journalArticle

Abstract

The occurrence of hospital-acquired acute gastroenteritis (AGE) is a major concern for public health. RotavirusA (RVA) and norovirus (NoV) are common causes of viral AGE in the pediatric population, and their role in nosocomial infections has been proven, remaining poorly investigated. To investigate RVA and NoV in hospital-acquired AGE, 55 stool samples from children with nosocomial AGE were collected between May 2014 and May 2015. To evaluate virus spreading routes, 51 environmental swabs were collected from staff and patients’ rooms. Stools were tested for both RVA and NoV RNA by reverse-transcription-PCR. Nucleotide sequencing and phylogenetic analysis were performed to characterize the viruses. Forty-seven of 55 cases analyzed resulted positive for RVA. The predominant genotype was G4P[8] (18/55) followed by G1P[8] (14/55). Mixed RVA infections were also detected (7/55). Twenty-two samples were positive for NoV, and GII.4 was revealed to be the predominant genotype. Seventeen samples were positive for both RVA and NoV. This study aimed to evaluate the burden of norovirus and rotavirus nosocomial AGE, contributing to identify the environment source of infections and to activate effective strategies for intervention. The reduction in nosocomial AGE cases is an important aspect, considered the worsened disease course in transplant, cancer, and intensive care unit inpatients.

Original languageEnglish
Pages (from-to)1768-1774
Number of pages7
JournalJournal of Medical Virology
Volume89
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Norovirus
Rotavirus
Gastroenteritis
Pediatrics
Hospital Oncology Service
Genotype
Viruses
Patients' Rooms
Cross Infection
Coinfection
Reverse Transcription
Intensive Care Units
Inpatients
Nucleotides
Public Health
RNA
Transplants
Polymerase Chain Reaction
Infection
Population

Keywords

  • gastroenteritis
  • human
  • Italy
  • norovirus
  • nosocomial
  • rotavirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Hospital-acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014-2015. / Valentini, Diletta; Ianiro, Giovanni; Di Bartolo, Ilaria; Di Camillo, Chiara; Boccuzzi, Elena; Vittucci, Anna C.; Ruggeri, Franco M.; Monini, Marina.

In: Journal of Medical Virology, Vol. 89, No. 10, 01.10.2017, p. 1768-1774.

Research output: Contribution to journalArticle

Valentini, Diletta ; Ianiro, Giovanni ; Di Bartolo, Ilaria ; Di Camillo, Chiara ; Boccuzzi, Elena ; Vittucci, Anna C. ; Ruggeri, Franco M. ; Monini, Marina. / Hospital-acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014-2015. In: Journal of Medical Virology. 2017 ; Vol. 89, No. 10. pp. 1768-1774.
@article{02630991960047d5a150341d3ef71f39,
title = "Hospital-acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014-2015",
abstract = "The occurrence of hospital-acquired acute gastroenteritis (AGE) is a major concern for public health. RotavirusA (RVA) and norovirus (NoV) are common causes of viral AGE in the pediatric population, and their role in nosocomial infections has been proven, remaining poorly investigated. To investigate RVA and NoV in hospital-acquired AGE, 55 stool samples from children with nosocomial AGE were collected between May 2014 and May 2015. To evaluate virus spreading routes, 51 environmental swabs were collected from staff and patients’ rooms. Stools were tested for both RVA and NoV RNA by reverse-transcription-PCR. Nucleotide sequencing and phylogenetic analysis were performed to characterize the viruses. Forty-seven of 55 cases analyzed resulted positive for RVA. The predominant genotype was G4P[8] (18/55) followed by G1P[8] (14/55). Mixed RVA infections were also detected (7/55). Twenty-two samples were positive for NoV, and GII.4 was revealed to be the predominant genotype. Seventeen samples were positive for both RVA and NoV. This study aimed to evaluate the burden of norovirus and rotavirus nosocomial AGE, contributing to identify the environment source of infections and to activate effective strategies for intervention. The reduction in nosocomial AGE cases is an important aspect, considered the worsened disease course in transplant, cancer, and intensive care unit inpatients.",
keywords = "gastroenteritis, human, Italy, norovirus, nosocomial, rotavirus",
author = "Diletta Valentini and Giovanni Ianiro and {Di Bartolo}, Ilaria and {Di Camillo}, Chiara and Elena Boccuzzi and Vittucci, {Anna C.} and Ruggeri, {Franco M.} and Marina Monini",
year = "2017",
month = "10",
day = "1",
doi = "10.1002/jmv.24866",
language = "English",
volume = "89",
pages = "1768--1774",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "10",

}

TY - JOUR

T1 - Hospital-acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014-2015

AU - Valentini, Diletta

AU - Ianiro, Giovanni

AU - Di Bartolo, Ilaria

AU - Di Camillo, Chiara

AU - Boccuzzi, Elena

AU - Vittucci, Anna C.

AU - Ruggeri, Franco M.

AU - Monini, Marina

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The occurrence of hospital-acquired acute gastroenteritis (AGE) is a major concern for public health. RotavirusA (RVA) and norovirus (NoV) are common causes of viral AGE in the pediatric population, and their role in nosocomial infections has been proven, remaining poorly investigated. To investigate RVA and NoV in hospital-acquired AGE, 55 stool samples from children with nosocomial AGE were collected between May 2014 and May 2015. To evaluate virus spreading routes, 51 environmental swabs were collected from staff and patients’ rooms. Stools were tested for both RVA and NoV RNA by reverse-transcription-PCR. Nucleotide sequencing and phylogenetic analysis were performed to characterize the viruses. Forty-seven of 55 cases analyzed resulted positive for RVA. The predominant genotype was G4P[8] (18/55) followed by G1P[8] (14/55). Mixed RVA infections were also detected (7/55). Twenty-two samples were positive for NoV, and GII.4 was revealed to be the predominant genotype. Seventeen samples were positive for both RVA and NoV. This study aimed to evaluate the burden of norovirus and rotavirus nosocomial AGE, contributing to identify the environment source of infections and to activate effective strategies for intervention. The reduction in nosocomial AGE cases is an important aspect, considered the worsened disease course in transplant, cancer, and intensive care unit inpatients.

AB - The occurrence of hospital-acquired acute gastroenteritis (AGE) is a major concern for public health. RotavirusA (RVA) and norovirus (NoV) are common causes of viral AGE in the pediatric population, and their role in nosocomial infections has been proven, remaining poorly investigated. To investigate RVA and NoV in hospital-acquired AGE, 55 stool samples from children with nosocomial AGE were collected between May 2014 and May 2015. To evaluate virus spreading routes, 51 environmental swabs were collected from staff and patients’ rooms. Stools were tested for both RVA and NoV RNA by reverse-transcription-PCR. Nucleotide sequencing and phylogenetic analysis were performed to characterize the viruses. Forty-seven of 55 cases analyzed resulted positive for RVA. The predominant genotype was G4P[8] (18/55) followed by G1P[8] (14/55). Mixed RVA infections were also detected (7/55). Twenty-two samples were positive for NoV, and GII.4 was revealed to be the predominant genotype. Seventeen samples were positive for both RVA and NoV. This study aimed to evaluate the burden of norovirus and rotavirus nosocomial AGE, contributing to identify the environment source of infections and to activate effective strategies for intervention. The reduction in nosocomial AGE cases is an important aspect, considered the worsened disease course in transplant, cancer, and intensive care unit inpatients.

KW - gastroenteritis

KW - human

KW - Italy

KW - norovirus

KW - nosocomial

KW - rotavirus

UR - http://www.scopus.com/inward/record.url?scp=85023195297&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85023195297&partnerID=8YFLogxK

U2 - 10.1002/jmv.24866

DO - 10.1002/jmv.24866

M3 - Article

AN - SCOPUS:85023195297

VL - 89

SP - 1768

EP - 1774

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 10

ER -