How to assess drug resistance in cancer stem cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-115
Number of pages9
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1692
ISSN (Print)1064-3745

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Keywords

  • Cancer stem cell culture
  • Colorectal cancer
  • CSC
  • Drug resistance
  • Drug screening
  • In vitro assays

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

De Angelis, M. L., De Maria, R., & Baiocchi, M. (2018). How to assess drug resistance in cancer stem cells. In Methods in Molecular Biology (pp. 107-115). (Methods in Molecular Biology; Vol. 1692). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7401-6_10