How to prepare cytological samples for molecular testing

Claudio Bellevicine, Umberto Malapelle, Elena Vigliar, Pasquale Pisapia, Giulia Vita, Giancarlo Troncone

Research output: Contribution to journalReview article

Abstract

This review is focused on the challenges in standardising and optimising molecular testing workflow in cytopathology. Although cytological samples yield optimal quality DNA, whose minimal amounts in most cases suffice even for multigene mutational profiling, the success of molecular testing is strongly dependent on standardised preanalytical protocols for maximising DNA yield and quality. Sample cytopreparation influences, even more, the quality of RNA and consequently the potential success of reverse transcription-PCR. Here, the educational and technical involvement of the cytopathologist as a relevant component of a multidisciplinary team, in the issues related to test request, specimen collection, fixation, processing, staining, tumour fraction enrichment, DNA quality/ quantity assessment and storage conditions is discussed. In addition, the specific sample requirements related to more recent technological developments are examined, underlining the modern role of the cytopathologist, whose continuous education is crucial to meet the opportunities of molecular medicine.

Original languageEnglish
Pages (from-to)819-826
Number of pages8
JournalJournal of Clinical Pathology
Volume70
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Bellevicine, C., Malapelle, U., Vigliar, E., Pisapia, P., Vita, G., & Troncone, G. (2017). How to prepare cytological samples for molecular testing. Journal of Clinical Pathology, 70(10), 819-826. https://doi.org/10.1136/jclinpath-2017-204561