HOX and TALE signatures specify human stromal stem cell populations from different sources

Jacopo Picchi, Luisa Trombi, Laura Spugnesi, Serena Barachini, Giorgia Maroni, Giovanni Barbanti Brodano, Stefano Boriani, Mauro Valtieri, Mario Petrini, Maria Cristina Magli

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Human stromal stem cell populations reside in different tissues and anatomical sites, however a critical question related to their efficient use in regenerative medicine is whether they exhibit equivalent biological properties. Here, we compared cellular and molecular characteristics of stromal stem cells derived from the bone marrow, at different body sites (iliac crest, sternum, and vertebrae) and other tissues (dental pulp and colon). In particular, we investigated whether homeobox genes of the HOX and TALE subfamilies might provide suitable markers to identify distinct stromal cell populations, as HOX proteins control cell positional identity and, together with their co-factors TALE, are involved in orchestrating differentiation of adult tissues. Our results show that stromal populations from different sources, although immunophenotypically similar, display distinct HOX and TALE signatures, as well as different growth and differentiation abilities. Stromal stem cells from different tissues are characterized by specific HOX profiles, differing in the number and type of active genes, as well as in their level of expression. Conversely, bone marrow-derived cell populations can be essentially distinguished for the expression levels of specific HOX members, strongly suggesting that quantitative differences in HOX activity may be crucial. Taken together, our data indicate that the HOX and TALE profiles provide positional, embryological and hierarchical identity of human stromal stem cells. Furthermore, our data suggest that cell populations derived from different body sites may not represent equivalent cell sources for cell-based therapeutical strategies for regeneration and repair of specific tissues.

Original languageEnglish
Pages (from-to)879-889
Number of pages11
JournalJournal of Cellular Physiology
Volume228
Issue number4
DOIs
Publication statusPublished - Apr 2013

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Stromal Cells
Stem cells
Stem Cells
Tissue
Population
Cells
Bone
Genes
Dental Pulp
Sternum
Regenerative Medicine
Homeobox Genes
Bone Marrow Cells
Pulp
Regeneration
Colon
Spine
Repair
Bone Marrow
Growth

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

HOX and TALE signatures specify human stromal stem cell populations from different sources. / Picchi, Jacopo; Trombi, Luisa; Spugnesi, Laura; Barachini, Serena; Maroni, Giorgia; Brodano, Giovanni Barbanti; Boriani, Stefano; Valtieri, Mauro; Petrini, Mario; Magli, Maria Cristina.

In: Journal of Cellular Physiology, Vol. 228, No. 4, 04.2013, p. 879-889.

Research output: Contribution to journalArticle

Picchi, J, Trombi, L, Spugnesi, L, Barachini, S, Maroni, G, Brodano, GB, Boriani, S, Valtieri, M, Petrini, M & Magli, MC 2013, 'HOX and TALE signatures specify human stromal stem cell populations from different sources', Journal of Cellular Physiology, vol. 228, no. 4, pp. 879-889. https://doi.org/10.1002/jcp.24239
Picchi, Jacopo ; Trombi, Luisa ; Spugnesi, Laura ; Barachini, Serena ; Maroni, Giorgia ; Brodano, Giovanni Barbanti ; Boriani, Stefano ; Valtieri, Mauro ; Petrini, Mario ; Magli, Maria Cristina. / HOX and TALE signatures specify human stromal stem cell populations from different sources. In: Journal of Cellular Physiology. 2013 ; Vol. 228, No. 4. pp. 879-889.
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