HPLC determination of selected amines with enzymatic biosensor and amperometric detection

Małgorzata Szewczyńska, Marzena Wcisło, Marek Trojanowicz, Jochen Saar, Enrico Dainese, Dario Compagnone

Research output: Contribution to journalArticle

Abstract

Diamine oxidase (DAO) obtained from lentil has been employed to the preparation of graphite paste amperometric biosensor, which has been utilised in the reversed phase HPLC system for detection of several amines: dopamine, benzylamine, benzylhydrazine and tyramine previously separated on various commercially available columns. Satisfactory results were obtained on Waters SymmetryShield RP8 column and monolithic Merck Chromolith column. The application of biosensor allows the selective detection of amines in complex blood serum matrix to be performed.

Original languageEnglish
Pages (from-to)591-606
Number of pages16
JournalChemia Analityczna
Volume48
Issue number3
Publication statusPublished - 2003

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Keywords

  • Amines
  • Amperometric biosensor
  • HPLC

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Szewczyńska, M., Wcisło, M., Trojanowicz, M., Saar, J., Dainese, E., & Compagnone, D. (2003). HPLC determination of selected amines with enzymatic biosensor and amperometric detection. Chemia Analityczna, 48(3), 591-606.