HPLC does not differentiate Mycobacterium paratuberculosis from Mycobacterium avium

R. Dei, E. Tortoli, A. Bartoloni, M. T. Simonetti, E. Lillini

Research output: Contribution to journalArticlepeer-review

Abstract

HPLC, which is gaining its place as identification tool in mycobacteriology laboratories, has been proposed to distinguish Mycobacterium paratuberculosis from Mycobacterium avium. We had reported no significant difference between M. avium and M. paratuberculosis reference strain ATCC 19698. Because of the advantages offered by such a method, we enlarged our observations to include more isolates of M. paratuberculosis. Within the double cluster of peaks obtained by both M. avium and M. paratuberculosis, we could not find a consistent difference typical of M. paratuberculosis. Therefore, the present study confirmed that M. avium and M. paratuberculosis could not be distinguished by HPLC, raising doubts of a straightforward use of HPLC to identify M. paratuberculosis. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalVeterinary Microbiology
Volume65
Issue number3
DOIs
Publication statusPublished - Mar 12 1999

Keywords

  • Diagnosis-bacteria
  • HPLC
  • Mycobacterium paratuberculosis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

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