TY - JOUR
T1 - HPLC-ESI-MS top-down analysis of salivary peptides of preterm newborns evidenced high activity of some exopeptidases and convertases during late fetal development
AU - Boroumand, Mozhgan
AU - Iavarone, Federica
AU - Manconi, Barbara
AU - Pieroni, Luisa
AU - Greco, Viviana
AU - Vento, Giovanni
AU - Tirone, Chiara
AU - Desiderio, Claudia
AU - Fiorita, Antonella
AU - Faa, Gavino
AU - Messana, Irene
AU - Cabras, Tiziana
AU - Olianas, Alessandra
AU - Castagnola, Massimo
N1 - Funding Information:
This work was financially supported by the Catholic University Intramural Research D1.1, by MIUR, Italy, and by the University of Cagliari (FIR-2017, TC; FIR-2016, AO ). We gratefully acknowledge the funding “Ricerca Corrente Neuroscienze Sperimentali (Cod. RC20. C – RC21. C) and the “Conto Capitale”CC- 2016–2365526 from the Italian Ministry of Health to IRCCS Fondazione Santa Lucia. LP and MC acknowledge the financial support 5 x 1000 (years 2017 and 2018) from Italian Ministry of Health to IRCCS Fondazione Santa Lucia. MB acknowledges ITPA and ITPA Foundations for travel grants support.
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8
Y1 - 2020/8
N2 - To have information on the proteolytic activity of convertases and exo-peptidases on human salivary proteins, this study investigated the relative amounts of the truncated proteoforms in the saliva of preterm newborns and compared them with the relative amounts measured in saliva of at-term newborns, of babies (0–10 years old) and of adults. Results indicated that convertase(s), acting on acidic proline-rich proteins and histatin 3, and carboxypeptidase(s) acting on acidic proline-rich proteins, P–C peptide, histatin 6 and statherin were many folds more active in preterm newborns than in the other groups. Conversely, the aminopeptidase responsible for the removal of the N-terminal Asp residue of statherin was not active in preterm newborns, becoming active only several months after the normal term of delivery. The high activity of convertases determined in preterm newborns suggests that it is required for the molecular events connected to the fetus development, and encourages further studies devoted to the characterization of their specific substrates.
AB - To have information on the proteolytic activity of convertases and exo-peptidases on human salivary proteins, this study investigated the relative amounts of the truncated proteoforms in the saliva of preterm newborns and compared them with the relative amounts measured in saliva of at-term newborns, of babies (0–10 years old) and of adults. Results indicated that convertase(s), acting on acidic proline-rich proteins and histatin 3, and carboxypeptidase(s) acting on acidic proline-rich proteins, P–C peptide, histatin 6 and statherin were many folds more active in preterm newborns than in the other groups. Conversely, the aminopeptidase responsible for the removal of the N-terminal Asp residue of statherin was not active in preterm newborns, becoming active only several months after the normal term of delivery. The high activity of convertases determined in preterm newborns suggests that it is required for the molecular events connected to the fetus development, and encourages further studies devoted to the characterization of their specific substrates.
KW - Histatins
KW - Newborns
KW - Proline-rich proteins
KW - Proteolysis
KW - Salivary proteins
KW - Statherin
UR - http://www.scopus.com/inward/record.url?scp=85090266998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85090266998&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2020.121429
DO - 10.1016/j.talanta.2020.121429
M3 - Article
C2 - 33167196
AN - SCOPUS:85090266998
VL - 222
JO - Talanta
JF - Talanta
SN - 0039-9140
M1 - 121429
ER -