HPLC monitoring of plasma catechol levels in the characterization of Parkinson's disease

P. Betto, G. Ricciarello, S. Ruggeri, F. Stocchi, F. Baronti, G. Nappi, E. Martignoni, G. V. Melzi D'Eril, F. Blandini, C. Lucarelli

Research output: Contribution to journalArticlepeer-review

Abstract

Peripheral factors, especially variations of plasma levels of L-DOPA and its catechol-related metabolites, seem to be the main cause of fluctuations during chronic treatment of Parkinson's disease. Over the past five years we have developed and refined some analytical chromatographic methods using HPLC with electrochemical and fluorimetric detection for the determination of L-DOPA and its major metabolites in plasma of Parkinsonian patients on L-DOPA therapy. HPLC-ECD analysis now is a powerful tool for the management of Parkinson's disease. Numerous chromatographic methods of analysis for L-DOPA and metabolites in biological fluids have been compared in this paper and the various analytical aspects have been examined. Clinical applications are also discussed. This short review includes unreported data concerning the utilization of HPLC methodologies to monitor plasma levels of Parkinsonian patients under pharmacological therapy with different drugs or ways of administration.

Original languageEnglish
Pages (from-to)441-453
Number of pages13
JournalGiornale Italiano di Chimica Clinica
Volume17
Issue number6
Publication statusPublished - 1992

Keywords

  • catechols
  • HPLC
  • L-DOPA
  • metabolites
  • Parkinson's disease

ASJC Scopus subject areas

  • Clinical Biochemistry

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