HPLC-tandem mass spectrometric method to characterize resveratrol metabolism in humans

Mireia Urpi-Sarda, Raul Zamora-Ros, Rosa Lamuela-Raventos, Antonio Cherubini, Olga Jauregui, Rafael De La Torre, Maria Isabel Covas, Ramon Estruch, Walter Jaeger, Cristina Andres-Lacueva

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Nutritional biomarkers are alternatives to traditional dietary assessment tools. We sought to develop a method for nutritional analysis of resveratrol, a phenolic compound with purported health-promoting properties, and to determine all resveratrol metabolites. Methods: We obtained LDL and urine samples from 11 healthy male volunteers who had consumed 250 mL of Merlot red wine. We measured resveratrol and its metabolites with 96-well solid-phase extraction plates coupled with HPLC-tandem mass spectrometry. Hexestrol was used as the internal standard. Gradient chromatography in multiple reaction monitoring mode was performed on a Luna C18 column, maintained at 40°C; mlz transitions were as follows: resveratrol, 227/185; resveratrol glucosides, 389/227; resveratrol glucuronides, 403/ 227; resveratrol sulfates, 307/227; taxifolin, 303/285; and hexestrol, 269/134. Results: Standard calibration curves were linear at 4.4-3289.5 nmol/L. Residual analyses were 100% (3.2) for trans-resveratrol and 100% (11.1) for trans-piceid. In both matrices, imprecision (CV) was

Original languageEnglish
Pages (from-to)292-299
Number of pages8
JournalClinical Chemistry
Volume53
Issue number2
DOIs
Publication statusPublished - Feb 2007

ASJC Scopus subject areas

  • Clinical Biochemistry

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