HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles

Sabrina De Carolis; Gianluca Storci; Claudio Ceccarelli; Claudia Savini; Lara Gallucci; Pasquale Sansone; Donatella Santini; Renato Seracchioli; Mario Taffurelli; Francesco Fabbri; Fabrizio Romani; Gaetano Compagnone; Cristina Giuliani; Paolo Garagnani; Massimiliano Bonafè; Monica Cricca

doi:10.3389/fonc.2019.00860

HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles

Sabrina De Carolis, Gianluca Storci, Claudio Ceccarelli, Claudia Savini, Lara Gallucci, Pasquale Sansone, Donatella Santini, Renato Seracchioli, Mario Taffurelli, Francesco Fabbri, Fabrizio Romani, Gaetano Compagnone, Cristina Giuliani, Paolo Garagnani, Massimiliano Bonafè, Monica Cricca

Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori

Research output: Contribution to journal › Article

Abstract

A causal link between Human Papillomavirus (HPV) and breast cancer (BC) remains controversial. In spite of this, the observation that HPV DNA is over-represented in the Triple Negative (TN) BC has been reported. Here we remark the high prevalence of HPV DNA (44.4%) in aggressive BC subtypes (TN and HER2+) in a population of 273 Italian women and we convey the presence of HPV DNA in the epithelial and stromal compartments by in situ hybridization. As previously reported, we also found that serum derived-extracellular vesicles (EVs) from BC affected patients contain HPV DNA. Interestingly, in one TNBC patient, the same HPV DNA type was detected in the serum-derived EVs, cervical and BC tissue samples. Then, we report that HPV DNA can be transferred by EVs to recipient BC stromal cells that show an activated phenotype (e.g., CD44, IL6 expression) and an enhanced capability to sustain mammospheres (MS) formation. These data suggest that HPV DNA vehiculated by EVs is a potential trigger for BC niche aggressiveness.

Original language	English
Article number	860
Journal	Frontiers in Oncology
Volume	9
DOIs	https://doi.org/10.3389/fonc.2019.00860
Publication status	Published - Sep 16 2019

Keywords

circulating HPV DNA
extracellular vesicles (EVs)
Human Papillomavirus (HPV)
stromal cells
triple negative BC

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.3389/fonc.2019.00860

Fingerprint Dive into the research topics of 'HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles'. Together they form a unique fingerprint.

Cite this

De Carolis, S., Storci, G., Ceccarelli, C., Savini, C., Gallucci, L., Sansone, P., Santini, D., Seracchioli, R., Taffurelli, M., Fabbri, F., Romani, F., Compagnone, G., Giuliani, C., Garagnani, P., Bonafè, M., & Cricca, M. (2019). HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles. Frontiers in Oncology, 9, [860]. https://doi.org/10.3389/fonc.2019.00860

HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles. / De Carolis, Sabrina; Storci, Gianluca; Ceccarelli, Claudio; Savini, Claudia; Gallucci, Lara; Sansone, Pasquale; Santini, Donatella; Seracchioli, Renato; Taffurelli, Mario; Fabbri, Francesco; Romani, Fabrizio; Compagnone, Gaetano; Giuliani, Cristina; Garagnani, Paolo; Bonafè, Massimiliano; Cricca, Monica.

In: Frontiers in Oncology, Vol. 9, 860, 16.09.2019.

Research output: Contribution to journal › Article

De Carolis, S, Storci, G, Ceccarelli, C, Savini, C, Gallucci, L, Sansone, P, Santini, D, Seracchioli, R, Taffurelli, M, Fabbri, F, Romani, F, Compagnone, G, Giuliani, C, Garagnani, P, Bonafè, M & Cricca, M 2019, 'HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles', Frontiers in Oncology, vol. 9, 860. https://doi.org/10.3389/fonc.2019.00860

De Carolis, Sabrina ; Storci, Gianluca ; Ceccarelli, Claudio ; Savini, Claudia ; Gallucci, Lara ; Sansone, Pasquale ; Santini, Donatella ; Seracchioli, Renato ; Taffurelli, Mario ; Fabbri, Francesco ; Romani, Fabrizio ; Compagnone, Gaetano ; Giuliani, Cristina ; Garagnani, Paolo ; Bonafè, Massimiliano ; Cricca, Monica. / HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles. In: Frontiers in Oncology. 2019 ; Vol. 9.

@article{f0d2a7d67b924a478894bec782e26491,

title = "HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles",

abstract = "A causal link between Human Papillomavirus (HPV) and breast cancer (BC) remains controversial. In spite of this, the observation that HPV DNA is over-represented in the Triple Negative (TN) BC has been reported. Here we remark the high prevalence of HPV DNA (44.4%) in aggressive BC subtypes (TN and HER2+) in a population of 273 Italian women and we convey the presence of HPV DNA in the epithelial and stromal compartments by in situ hybridization. As previously reported, we also found that serum derived-extracellular vesicles (EVs) from BC affected patients contain HPV DNA. Interestingly, in one TNBC patient, the same HPV DNA type was detected in the serum-derived EVs, cervical and BC tissue samples. Then, we report that HPV DNA can be transferred by EVs to recipient BC stromal cells that show an activated phenotype (e.g., CD44, IL6 expression) and an enhanced capability to sustain mammospheres (MS) formation. These data suggest that HPV DNA vehiculated by EVs is a potential trigger for BC niche aggressiveness.",

keywords = "circulating HPV DNA, extracellular vesicles (EVs), Human Papillomavirus (HPV), stromal cells, triple negative BC",

author = "{De Carolis}, Sabrina and Gianluca Storci and Claudio Ceccarelli and Claudia Savini and Lara Gallucci and Pasquale Sansone and Donatella Santini and Renato Seracchioli and Mario Taffurelli and Francesco Fabbri and Fabrizio Romani and Gaetano Compagnone and Cristina Giuliani and Paolo Garagnani and Massimiliano Bonaf{\`e} and Monica Cricca",

year = "2019",

month = sep,

day = "16",

doi = "10.3389/fonc.2019.00860",

language = "English",

volume = "9",

journal = "Frontiers in Oncology",

issn = "2234-943X",

publisher = "Frontiers Media S. A.",

}

TY - JOUR

T1 - HPV DNA Associates With Breast Cancer Malignancy and It Is Transferred to Breast Cancer Stromal Cells by Extracellular Vesicles

AU - De Carolis, Sabrina

AU - Storci, Gianluca

AU - Ceccarelli, Claudio

AU - Savini, Claudia

AU - Gallucci, Lara

AU - Sansone, Pasquale

AU - Santini, Donatella

AU - Seracchioli, Renato

AU - Taffurelli, Mario

AU - Fabbri, Francesco

AU - Romani, Fabrizio

AU - Compagnone, Gaetano

AU - Giuliani, Cristina

AU - Garagnani, Paolo

AU - Bonafè, Massimiliano

AU - Cricca, Monica

PY - 2019/9/16

Y1 - 2019/9/16

N2 - A causal link between Human Papillomavirus (HPV) and breast cancer (BC) remains controversial. In spite of this, the observation that HPV DNA is over-represented in the Triple Negative (TN) BC has been reported. Here we remark the high prevalence of HPV DNA (44.4%) in aggressive BC subtypes (TN and HER2+) in a population of 273 Italian women and we convey the presence of HPV DNA in the epithelial and stromal compartments by in situ hybridization. As previously reported, we also found that serum derived-extracellular vesicles (EVs) from BC affected patients contain HPV DNA. Interestingly, in one TNBC patient, the same HPV DNA type was detected in the serum-derived EVs, cervical and BC tissue samples. Then, we report that HPV DNA can be transferred by EVs to recipient BC stromal cells that show an activated phenotype (e.g., CD44, IL6 expression) and an enhanced capability to sustain mammospheres (MS) formation. These data suggest that HPV DNA vehiculated by EVs is a potential trigger for BC niche aggressiveness.

AB - A causal link between Human Papillomavirus (HPV) and breast cancer (BC) remains controversial. In spite of this, the observation that HPV DNA is over-represented in the Triple Negative (TN) BC has been reported. Here we remark the high prevalence of HPV DNA (44.4%) in aggressive BC subtypes (TN and HER2+) in a population of 273 Italian women and we convey the presence of HPV DNA in the epithelial and stromal compartments by in situ hybridization. As previously reported, we also found that serum derived-extracellular vesicles (EVs) from BC affected patients contain HPV DNA. Interestingly, in one TNBC patient, the same HPV DNA type was detected in the serum-derived EVs, cervical and BC tissue samples. Then, we report that HPV DNA can be transferred by EVs to recipient BC stromal cells that show an activated phenotype (e.g., CD44, IL6 expression) and an enhanced capability to sustain mammospheres (MS) formation. These data suggest that HPV DNA vehiculated by EVs is a potential trigger for BC niche aggressiveness.

KW - circulating HPV DNA

KW - extracellular vesicles (EVs)

KW - Human Papillomavirus (HPV)

KW - stromal cells

KW - triple negative BC

UR - http://www.scopus.com/inward/record.url?scp=85072881153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072881153&partnerID=8YFLogxK

U2 - 10.3389/fonc.2019.00860

DO - 10.3389/fonc.2019.00860

M3 - Article

AN - SCOPUS:85072881153

VL - 9

JO - Frontiers in Oncology

JF - Frontiers in Oncology

SN - 2234-943X

M1 - 860

ER -