HPV self-sampling in CIN2+ detection

Sensitivity and specificity of different RLU cut-off of HC2 in specimens from 786 women

F. Bottari, S. Igidbashian, S. Boveri, A. Tricca, C. Gulmini, M. Sesia, N. Spolti, M. Sideri, F. Landoni, M. T. Sandri

Research output: Contribution to journalArticle

Abstract

Aims Mortality for cervical cancer varies between the different regions of the world, with high rates in low-income countries where screening programmes are not present and organised. However, increasing screening coverage is still a priority in all countries: one way to do that is to base screening on self-sampled screening. The success of a self-sampling screening strategy depends on capacity to recruit unscreened women, on the performance and acceptability of the device and on the clinical performance of the high-risk human papillomavirus (HPV) test. Methods This study based on 786 enrolled women investigates the best cut-off value of Hybrid Capture 2 HPV test (HC2) for self-sampled specimens in terms of sensitivity and specificity. Results In this population, we found that the sensitivity and the specificity for cervical intraepithelial neoplasia grade 2 or more detection of HC2 performed on self-sampled specimens were 82.5% and 82.8%, respectively considering the relative light units (RLU) cut-off value of 1. Increasing the cut-off value the sensitivity decreases and the specificity raises and the best area under the curve for the RLU cut-off value is 1. Conclusions Our results confirm that the cut-off value of 1 suggested by Qiagen for PreservCyt specimen is the best cut-off value also for self-sampled specimens.

Original languageEnglish
Pages (from-to)327-330
Number of pages4
JournalJournal of Clinical Pathology
Volume70
Issue number4
DOIs
Publication statusPublished - Apr 1 2017

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Light
Sensitivity and Specificity
Cervical Intraepithelial Neoplasia
Uterine Cervical Neoplasms
Area Under Curve
Equipment and Supplies
Mortality
Population

Keywords

  • CERVICAL CANCER
  • HPV
  • LABORATORY TESTS

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

HPV self-sampling in CIN2+ detection : Sensitivity and specificity of different RLU cut-off of HC2 in specimens from 786 women. / Bottari, F.; Igidbashian, S.; Boveri, S.; Tricca, A.; Gulmini, C.; Sesia, M.; Spolti, N.; Sideri, M.; Landoni, F.; Sandri, M. T.

In: Journal of Clinical Pathology, Vol. 70, No. 4, 01.04.2017, p. 327-330.

Research output: Contribution to journalArticle

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