Hsp60 response in experimental and human temporal lobe epilepsy

Antonella Marino Gammazza, Roberto Colangeli, Gergely Orban, Massimo Pierucci, Giancarlo Di Gennaro, Margherita Lo Bello, Alfredo D'Aniello, Fabio Bucchieri, Cristoforo Pomara, Mario Valentino, Richard Muscat, Arcangelo Benigno, Giovanni Zummo, Everly C onway de Macario, Francesco Cappello, Giuseppe Di Giovanni, Alberto J L Macario

Research output: Contribution to journalArticlepeer-review


The mitochondrial chaperonin Hsp60 is a ubiquitous molecule with multiple roles, constitutively expressed and inducible by oxidative stress. In the brain, Hsp60 is widely distributed and has been implicated in neurological disorders, including epilepsy. A role for mitochondria and oxidative stress has been proposed in epileptogenesis of temporal lobe epilepsy (TLE). Here, we investigated the involvement of Hsp60 in TLE using animal and human samples. Hsp60 immunoreactivity in the hippocampus, measured by Western blotting and immunohistochemistry, was increased in a rat model of TLE. Hsp60 was also increased in the hippocampal dentate gyrus neurons somata and neuropil and hippocampus proper (CA3, CA1) of the epileptic rats. We also determined the circulating levels of Hsp60 in epileptic animals and TLE patients using ELISA. The epileptic rats showed circulating levels of Hsp60 higher than controls. Likewise, plasma post-seizure Hsp60 levels in patients were higher than before the seizure and those of controls. These results demonstrate that Hsp60 is increased in both animals and patients with TLE in affected tissues, and in plasma in response to epileptic seizures, and point to it as biomarker of hippocampal stress potentially useful for diagnosis and patient management.

Original languageEnglish
Pages (from-to)9434
Number of pages1
JournalScientific Reports
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Hsp60 response in experimental and human temporal lobe epilepsy'. Together they form a unique fingerprint.

Cite this