HT-29 cells: a new substrate for rotavirus growth

F. Superti, A. Tinari, L. Baldassarri, G. Donelli

Research output: Contribution to journalArticle

Abstract

Susceptibilities of a continuous rhesus monkey kidney cell line (MA-104) and that of a human colon carcinoma cell line (HT-29) to infection by different human and animal rotavirus strains were compared. HT-29 cells appeared to be more sensitive to human rotavirus infection than MA-104 cells, whereas the latter cell line was more susceptible to animal rotavirus replication. The greater sensitivity to human rotavirus infection of HT-29 cells was confirmed by the successful, direct isolation of these viruses from faecal specimens. Human rotavirus infection of HT-29 cells was also followed by transmission electron microscopy. In ultra-thin sections, unenveloped particles of rotaviruses, representing infectious mature virions, were observed in large number. Moreover, many "double-shelled" particles were detected in negative-stained supernatants from infected cultures. Scanning electron microscopy of uninfected HT-29 cells showed that in the presence of Ca+ +, required for rotavirus growth, they are able to express some of the features of mature intestinal cells. In view of these results, HT-29 cells appear to be a useful in vitro model for the study of rotavirus infection.

Original languageEnglish
Pages (from-to)159-173
Number of pages15
JournalArchives of Virology
Volume116
Issue number1-4
DOIs
Publication statusPublished - Mar 1991

Fingerprint

HT29 Cells
Rotavirus
Rotavirus Infections
Growth
Cell Line
Macaca mulatta
Transmission Electron Microscopy
Virion
Electron Scanning Microscopy
Colon
Viruses
Carcinoma
Kidney
Infection

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Superti, F., Tinari, A., Baldassarri, L., & Donelli, G. (1991). HT-29 cells: a new substrate for rotavirus growth. Archives of Virology, 116(1-4), 159-173. https://doi.org/10.1007/BF01319239

HT-29 cells : a new substrate for rotavirus growth. / Superti, F.; Tinari, A.; Baldassarri, L.; Donelli, G.

In: Archives of Virology, Vol. 116, No. 1-4, 03.1991, p. 159-173.

Research output: Contribution to journalArticle

Superti, F, Tinari, A, Baldassarri, L & Donelli, G 1991, 'HT-29 cells: a new substrate for rotavirus growth', Archives of Virology, vol. 116, no. 1-4, pp. 159-173. https://doi.org/10.1007/BF01319239
Superti F, Tinari A, Baldassarri L, Donelli G. HT-29 cells: a new substrate for rotavirus growth. Archives of Virology. 1991 Mar;116(1-4):159-173. https://doi.org/10.1007/BF01319239
Superti, F. ; Tinari, A. ; Baldassarri, L. ; Donelli, G. / HT-29 cells : a new substrate for rotavirus growth. In: Archives of Virology. 1991 ; Vol. 116, No. 1-4. pp. 159-173.
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