Abstract
Susceptibilities of a continuous rhesus monkey kidney cell line (MA-104) and that of a human colon carcinoma cell line (HT-29) to infection by different human and animal rotavirus strains were compared. HT-29 cells appeared to be more sensitive to human rotavirus infection than MA-104 cells, whereas the latter cell line was more susceptible to animal rotavirus replication. The greater sensitivity to human rotavirus infection of HT-29 cells was confirmed by the successful, direct isolation of these viruses from faecal specimens. Human rotavirus infection of HT-29 cells was also followed by transmission electron microscopy. In ultra-thin sections, unenveloped particles of rotaviruses, representing infectious mature virions, were observed in large number. Moreover, many "double-shelled" particles were detected in negative-stained supernatants from infected cultures. Scanning electron microscopy of uninfected HT-29 cells showed that in the presence of Ca+ +, required for rotavirus growth, they are able to express some of the features of mature intestinal cells. In view of these results, HT-29 cells appear to be a useful in vitro model for the study of rotavirus infection.
| Original language | English |
|---|---|
| Pages (from-to) | 159-173 |
| Number of pages | 15 |
| Journal | Archives of Virology |
| Volume | 116 |
| Issue number | 1-4 |
| DOIs | |
| Publication status | Published - Mar 1991 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Genetics
Cite this
HT-29 cells : a new substrate for rotavirus growth. / Superti, F.; Tinari, A.; Baldassarri, L.; Donelli, G.
In: Archives of Virology, Vol. 116, No. 1-4, 03.1991, p. 159-173.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HT-29 cells
T2 - a new substrate for rotavirus growth
AU - Superti, F.
AU - Tinari, A.
AU - Baldassarri, L.
AU - Donelli, G.
PY - 1991/3
Y1 - 1991/3
N2 - Susceptibilities of a continuous rhesus monkey kidney cell line (MA-104) and that of a human colon carcinoma cell line (HT-29) to infection by different human and animal rotavirus strains were compared. HT-29 cells appeared to be more sensitive to human rotavirus infection than MA-104 cells, whereas the latter cell line was more susceptible to animal rotavirus replication. The greater sensitivity to human rotavirus infection of HT-29 cells was confirmed by the successful, direct isolation of these viruses from faecal specimens. Human rotavirus infection of HT-29 cells was also followed by transmission electron microscopy. In ultra-thin sections, unenveloped particles of rotaviruses, representing infectious mature virions, were observed in large number. Moreover, many "double-shelled" particles were detected in negative-stained supernatants from infected cultures. Scanning electron microscopy of uninfected HT-29 cells showed that in the presence of Ca+ +, required for rotavirus growth, they are able to express some of the features of mature intestinal cells. In view of these results, HT-29 cells appear to be a useful in vitro model for the study of rotavirus infection.
AB - Susceptibilities of a continuous rhesus monkey kidney cell line (MA-104) and that of a human colon carcinoma cell line (HT-29) to infection by different human and animal rotavirus strains were compared. HT-29 cells appeared to be more sensitive to human rotavirus infection than MA-104 cells, whereas the latter cell line was more susceptible to animal rotavirus replication. The greater sensitivity to human rotavirus infection of HT-29 cells was confirmed by the successful, direct isolation of these viruses from faecal specimens. Human rotavirus infection of HT-29 cells was also followed by transmission electron microscopy. In ultra-thin sections, unenveloped particles of rotaviruses, representing infectious mature virions, were observed in large number. Moreover, many "double-shelled" particles were detected in negative-stained supernatants from infected cultures. Scanning electron microscopy of uninfected HT-29 cells showed that in the presence of Ca+ +, required for rotavirus growth, they are able to express some of the features of mature intestinal cells. In view of these results, HT-29 cells appear to be a useful in vitro model for the study of rotavirus infection.
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U2 - 10.1007/BF01319239
DO - 10.1007/BF01319239
M3 - Article
C2 - 1848062
AN - SCOPUS:0026071999
VL - 116
SP - 159
EP - 173
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 1-4
ER -