HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α

Claudio Casoli, Elisa Vicenzi, Andrea Cimarelli, Giacomo Magnani, Paolo Ciancianaini, Ercole Cattaneo, PierPaolo Dall'Aglio, Guido Poli, Umberto Bertazzoni

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Abstract

The influence of human T-cell leukemia/lymphoma virus type II (HTLV-II) in individuals also infected with HIV-1 is poorly understood. To evaluate the reciprocal influence of HTLV-II and HIV-1 infection, primary peripheral blood mononuclear cell (PBMC) cultures from coinfected individuals were established in the presence of interleukin 2 (IL-2). In these cultures, the kinetics of HTLV-II replication always preceded those of HIV-1. Noteworthy, the kinetics of HIV-1 production were inversely correlated to the HTLV-II proviral load in vivo and its replication ex vivo. These observations suggested a potential interaction between the 2 retroviruses. In this regard, the levels of IL-2, IL-6, and tumor necrosis factor-α (TNF-α) were measured in the same coinfected PBMC cultures. Endogenous IL-2 was not produced, whereas IL-6 and TNF-α were secreted at levels compatible with their known ability to up- regulate HIV-1 expression. The HIV-suppressive CC-chemokines RANTES, macrophage inflammatory protein-1α (MIP-1α), and MIP-1β were also determined in IL-2-stimulated PBMC cultures. Of interest, their kinetics and concentrations were inversely related to those of HIV-1 replication. Experiments were performed in which CD8+ T cells or PBMCs from HTLV-II monoinfected individuals were cocultivated with CD4+ T cells from HIV-1 monoinfected individuals separated by a semipermeable membrane in the presence or absence of antichemokine neutralizing antibodies. The results indicate that HTLV-II can interfere with the replicative potential of HIV-1 by up-regulating viral suppressive CC-chemokines and, in particular, MIP-1α. This study is the first report indicating that HTLV-II can influence HIV replication, at least in vitro, via up-regulation of HIV-suppressive chemokines. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)2760-2769
Number of pages10
JournalBlood
Volume95
Issue number9
Publication statusPublished - May 1 2000

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Human T-lymphotropic virus 2
Deltaretrovirus
Macrophage Inflammatory Proteins
T-cells
Viruses
Interleukin-2
HIV-1
Blood Cells
Blood
Down-Regulation
Cell culture
CC Chemokines
Cell Culture Techniques
HIV
Interleukin-6
Kinetics
Up-Regulation
Tumor Necrosis Factor-alpha
T-Lymphocytes
Chemokine CCL5

ASJC Scopus subject areas

  • Hematology

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Casoli, C., Vicenzi, E., Cimarelli, A., Magnani, G., Ciancianaini, P., Cattaneo, E., ... Bertazzoni, U. (2000). HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α. Blood, 95(9), 2760-2769.

HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α. / Casoli, Claudio; Vicenzi, Elisa; Cimarelli, Andrea; Magnani, Giacomo; Ciancianaini, Paolo; Cattaneo, Ercole; Dall'Aglio, PierPaolo; Poli, Guido; Bertazzoni, Umberto.

In: Blood, Vol. 95, No. 9, 01.05.2000, p. 2760-2769.

Research output: Contribution to journalArticle

Casoli, C, Vicenzi, E, Cimarelli, A, Magnani, G, Ciancianaini, P, Cattaneo, E, Dall'Aglio, P, Poli, G & Bertazzoni, U 2000, 'HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α', Blood, vol. 95, no. 9, pp. 2760-2769.
Casoli C, Vicenzi E, Cimarelli A, Magnani G, Ciancianaini P, Cattaneo E et al. HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α. Blood. 2000 May 1;95(9):2760-2769.
Casoli, Claudio ; Vicenzi, Elisa ; Cimarelli, Andrea ; Magnani, Giacomo ; Ciancianaini, Paolo ; Cattaneo, Ercole ; Dall'Aglio, PierPaolo ; Poli, Guido ; Bertazzoni, Umberto. / HTLV-II down-regulates HIV-1 replication in IL-2-stimulated primary PBMC of coinfected individuals through expression of MIP-1α. In: Blood. 2000 ; Vol. 95, No. 9. pp. 2760-2769.
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AU - Ciancianaini, Paolo

AU - Cattaneo, Ercole

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