HTLV infection among Italian intravenous drug users and North African subjects detected by the polymerase chain reaction and serological methods

E. Colombo, I. Magistrem, E. Mendozzi, E. Cattaneo, G. Achilli, P. Ferrante

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Six hundred intravenous drug users (IVDUs) and two hundred North Africans were screened for human T-cell leukemia virus (HTLV) antibodies using several serological methods. Eighteen of the eighty-two HTLV-seropositive individuals were also tested by the polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of immunoenzymatic detection of the amplified DNA. Of these eighteen subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA, whereas all of the eighteen subjects were negative for HTLV-I. Western blot (WE) confirmed six of the eight HTLV-positive subjects, while the results of the remaining two were indeterminate. The results confirmed the PCR-DEIA as a rapid and an efficient method of discriminating between HTLV-I and HTLV-II infection, whereas serological tests, including the WE, have limitations in terms of specificity and sensitivity. Moreover, this study showed a higher frequency of HTLV seroreactivity in the Italian IVDU population than in previous studies and confirmed that HTLV-II is more frequent than HTLV-I in this population.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalJournal of Medical Virology
Volume47
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Deltaretrovirus
Human T-lymphotropic virus 2
Virus Diseases
Drug Users
Human T-lymphotropic virus 1
Immunoenzyme Techniques
Polymerase Chain Reaction
DNA
Deltaretrovirus Antibodies
Western Blotting
Serologic Tests
Population
Sensitivity and Specificity

Keywords

  • DNA amplification
  • HTLV-I/II differentiation
  • Human retrovirus
  • Risk groups
  • Serology

ASJC Scopus subject areas

  • Virology

Cite this

HTLV infection among Italian intravenous drug users and North African subjects detected by the polymerase chain reaction and serological methods. / Colombo, E.; Magistrem, I.; Mendozzi, E.; Cattaneo, E.; Achilli, G.; Ferrante, P.

In: Journal of Medical Virology, Vol. 47, No. 1, 1995, p. 10-15.

Research output: Contribution to journalArticle

@article{d8d3647a75d6467e97876aa2aab4162f,
title = "HTLV infection among Italian intravenous drug users and North African subjects detected by the polymerase chain reaction and serological methods",
abstract = "Six hundred intravenous drug users (IVDUs) and two hundred North Africans were screened for human T-cell leukemia virus (HTLV) antibodies using several serological methods. Eighteen of the eighty-two HTLV-seropositive individuals were also tested by the polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of immunoenzymatic detection of the amplified DNA. Of these eighteen subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA, whereas all of the eighteen subjects were negative for HTLV-I. Western blot (WE) confirmed six of the eight HTLV-positive subjects, while the results of the remaining two were indeterminate. The results confirmed the PCR-DEIA as a rapid and an efficient method of discriminating between HTLV-I and HTLV-II infection, whereas serological tests, including the WE, have limitations in terms of specificity and sensitivity. Moreover, this study showed a higher frequency of HTLV seroreactivity in the Italian IVDU population than in previous studies and confirmed that HTLV-II is more frequent than HTLV-I in this population.",
keywords = "DNA amplification, HTLV-I/II differentiation, Human retrovirus, Risk groups, Serology",
author = "E. Colombo and I. Magistrem and E. Mendozzi and E. Cattaneo and G. Achilli and P. Ferrante",
year = "1995",
doi = "10.1002/jmv.1890470104",
language = "English",
volume = "47",
pages = "10--15",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - HTLV infection among Italian intravenous drug users and North African subjects detected by the polymerase chain reaction and serological methods

AU - Colombo, E.

AU - Magistrem, I.

AU - Mendozzi, E.

AU - Cattaneo, E.

AU - Achilli, G.

AU - Ferrante, P.

PY - 1995

Y1 - 1995

N2 - Six hundred intravenous drug users (IVDUs) and two hundred North Africans were screened for human T-cell leukemia virus (HTLV) antibodies using several serological methods. Eighteen of the eighty-two HTLV-seropositive individuals were also tested by the polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of immunoenzymatic detection of the amplified DNA. Of these eighteen subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA, whereas all of the eighteen subjects were negative for HTLV-I. Western blot (WE) confirmed six of the eight HTLV-positive subjects, while the results of the remaining two were indeterminate. The results confirmed the PCR-DEIA as a rapid and an efficient method of discriminating between HTLV-I and HTLV-II infection, whereas serological tests, including the WE, have limitations in terms of specificity and sensitivity. Moreover, this study showed a higher frequency of HTLV seroreactivity in the Italian IVDU population than in previous studies and confirmed that HTLV-II is more frequent than HTLV-I in this population.

AB - Six hundred intravenous drug users (IVDUs) and two hundred North Africans were screened for human T-cell leukemia virus (HTLV) antibodies using several serological methods. Eighteen of the eighty-two HTLV-seropositive individuals were also tested by the polymerase chain reaction-DNA enzyme immunoassay (PCR-DEIA), a non-isotopic method of immunoenzymatic detection of the amplified DNA. Of these eighteen subjects, eight IVDUs were found to be HTLV-II-positive by the PCR-DEIA, whereas all of the eighteen subjects were negative for HTLV-I. Western blot (WE) confirmed six of the eight HTLV-positive subjects, while the results of the remaining two were indeterminate. The results confirmed the PCR-DEIA as a rapid and an efficient method of discriminating between HTLV-I and HTLV-II infection, whereas serological tests, including the WE, have limitations in terms of specificity and sensitivity. Moreover, this study showed a higher frequency of HTLV seroreactivity in the Italian IVDU population than in previous studies and confirmed that HTLV-II is more frequent than HTLV-I in this population.

KW - DNA amplification

KW - HTLV-I/II differentiation

KW - Human retrovirus

KW - Risk groups

KW - Serology

UR - http://www.scopus.com/inward/record.url?scp=0029074878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029074878&partnerID=8YFLogxK

U2 - 10.1002/jmv.1890470104

DO - 10.1002/jmv.1890470104

M3 - Article

C2 - 8551251

AN - SCOPUS:0029074878

VL - 47

SP - 10

EP - 15

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 1

ER -