Human α-thrombin induces phosphoinositide turnover and Ca2+ movements in cultured human umbilical vein endothelial cells

M. G. Lampugnani, M. Pedenovi, E. Dejana, D. Rotilio, M. B. Donati, F. Bussolino, G. Garbarino, D. Ghigo, A. Bosia

Research output: Contribution to journalArticle

Abstract

This report shows that purified human α-thrombin was able to stimulate a rapid and transient formation of water-soluble phosphorylated 3H-inositols in cultured human umbilical vein endothelial cells (HUVEC) prelabelled with 3H-inositol. A parallel breakdown and resynthesis of 3H-inositol-containing phospholipids was observed. Simultaneously, thrombin induced a transient increase of intracellular free Ca2+([Ca2+]1), as measured from increased fluorescence of quin2 loaded cells. Phosphoinositide turnover and Ca2+ mobilization showed a similar dependence on thrombin dose. [Ca2+]1 rise resulted from both influx from extraceilular medium and redistribution from intracellular storage sites. On the other hand thrombin-induced phosphoinositide hydrolysis was not dependent on [Ca2+]1 rise. [Ca2+]1 elevation might be, at least partially, a consequence of increased phosphoinositide turnover, as suggested by [Ca2+]-mobilizing activity of inositol-trisphosphate in other cells.

Original languageEnglish
Pages (from-to)75-87
Number of pages13
JournalThrombosis Research
Volume54
Issue number1
DOIs
Publication statusPublished - Apr 1 1989

Keywords

  • Ca movements
  • human umbilical vein endothelial cells
  • phosphoinositide turnover
  • α-thrombin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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