Human μ opiate receptor. cDNA and genomic clones, pharmacologic characterization and chromosomal assignment

Jia Bei Wang, Peter S. Johnson, Antonio M. Persico, Anita L. Hawkins, Constance A. Griffin, George R. Uhl

Research output: Contribution to journalArticlepeer-review

Abstract

A human μ opiate receptor cDNA has been identified from a cerebral cortical cDNA library using sequences from the rat μ opiate receptor cDNA. The human μ opiate receptor (hμOR1) shares 95% amino acid identity with the rat sequence. The expressed μOR1 recognizes tested opiate drugs and opioid peptides in a sodium- and GTP-sensitive fashion with affinities virtually identical to those displayed by the rat μ opiate receptor. Effects on cyclic AMP are similar to those noted for the rat μ opiate receptor. An 18 kb genomic clone hybridizing with the hμOR1 cDNA contains 63 and 489 bp exonic sequences flanked by splice donor/acceptor sequences. Analysis of hybridization to DNA prepared from human rodent hybrid cell lines and chromosomal in situ hybridization studies indicate localization to 6q24-25. An MspI polymorphism, producing a 3.7 kb band, may prove useful in assessing this gene's involvement in neuropsychiatric disorders involving opiatergic systems.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalFEBS Letters
Volume338
Issue number2
DOIs
Publication statusPublished - Jan 31 1994

Keywords

  • Addiction
  • Enkephalin
  • G-linked receptor
  • Heroin
  • Morphine
  • Pain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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