Human acetyl-CoA carboxylase: Characterization, molecular cloning, and evidence for two isoforms

L. Abu-Elheiga, A. Jayakumar, A. Baldini, S. S. Chirala, S. J. Wakil

Research output: Contribution to journalArticle

Abstract

We have cloned and sequenced the cDNA coding for human HepG2 acetyl-CoA carboxylase (ACC; EC 6.4.1.2). The sequence has an open reading frame of 7038 bp that encode 2346 amino acids (M(r). 264,737). The C-terminal 2.6-kb sequence is very different from that recently reported for human ACC (Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. and Kim, K.-H. [1994] Eur. J. Biochem. 219, 297-306). Northern blot analysis revealed that the ACC mRNA is ≃10 kb in size and that its level varies among the tissues tested. Evidence is presented to show that the human ACC gene is 200-480 kbp in size and maps to chromosome 17q12. We also provide evidence for the presence of another ACC-like gene with similarly sized mRNA but tissue-specific expression different from that of the ACC gene reported herein. That this second ACC-like gene encodes the 280-kDa carboxylase is not ruled out.

Original languageEnglish
Pages (from-to)4011-4015
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number9
Publication statusPublished - 1995

ASJC Scopus subject areas

  • General
  • Genetics

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