Human acute leukemia cells injected in NOD/LtSz-scid/IL-2Rγ null mice generate a faster and more efficient disease compared to other NOD/scid-related strains

Alice Agliano, Ines Martin-Padura, Patrizia Mancuso, Paola Marighetti, Cristina Rabascio, Giancarlo Pruneri, Leonard D. Shultz, Francesco Bertolini

Research output: Contribution to journalArticle

Abstract

Transplantation of human acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) primary cells and cell lines in different strains of immunodeficient mice has led to preclinical models extensively used to investigate acute leukemia stem cells, biology and drug sensitivity. We studied the engraftment kinetics of AML and ALL cell lines and primary cells in 3 strains of NOD.CB17-Prkdcscid (NOD/scid, NS)-related mice (NOD.Cg-PrkdcscidB2mtm1Unc/J, abbreviated NOD/scid/β2 null, NSB; and NOD.Cg-PrkdcscidIl2rgtmIWjll/SzJ, abbreviated NOD/scid/IL-2Rγ null, NSG). The engraftment of human malignant cells was investigated by means of clinicopathological criteria, flow cytometry, PCR and immunohistochemistry. In NSG mice, we observed a significantly faster development of leukemia-related symptoms and a higher percentage of leukemia cells in the blood, in the marrow and in the spleen. The leukemia-related angiogenic switch (measured as the number of circulating endothelial cells and progenitors) was faster in NSG compared to NS and NSB mice. These models will be instrumental to studies on leukemia-initiating stem cells, leukemia biology, preclinical treatment studies, and to obtain patient-specific preclinical models to design and investigate patient-tailored therapies.

Original languageEnglish
Pages (from-to)2222-2227
Number of pages6
JournalInternational Journal of Cancer
Volume123
Issue number9
DOIs
Publication statusPublished - Nov 1 2008

Keywords

  • Acute leukemia
  • Circulating endothelial progenitors
  • Preclinical models

ASJC Scopus subject areas

  • Cancer Research
  • Medicine(all)
  • Oncology

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