Human adipose-derived stem cells (hASCs) proliferate and differentiate in osteoblast-like cells on trabecular titanium scaffolds

Giulia Gastaldi, Annalia Asti, Manuela Federica Scaffino, Livia Visai, Enrica Saino, Angela Maria Cometa, Francesco Benazzo

Research output: Contribution to journalArticle

Abstract

The use of stem cells in regenerative medicine is an appealing area of research that has received a great deal of interest in recent years. The population called human adipose tissue-derived stem cells (hASCs) share many of the characteristic of its counterpart of marrow including extensive proliferative potential and the ability to undergo multilineage differentiation along classical mesenchymal lineages: adipogenesis, chondrogenesis, osteogenesis, and myogenesis. The aim of this study was to evaluate with biochemical and morphological methods the adhesion and differentiation of hASCs grown on trabecular titanium scaffolds. The hASCs isolated from subcutaneous adipose tissue after digestion with collagenase were seeded on monolayer and on trabecular titanium scaffolds and incubated at 37°C in 5% CO2 with osteogenic medium or control medium.-The results showed that hASCs were able to adhere to titanium scaffolds, to proliferate, to acquire an osteoblastic-like phenotype, and to produce a calcified extracellular matrix with protein, such as, decorin, fibronectin, osteocalcin, osteonectin, osteopontin, and type I collagen. These data suggest that this kind of scaffold/cells construct is effective to regenerate damaged tissue and to restore the function of bone tissue.

Original languageEnglish
Pages (from-to)790-799
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume94
Issue number3
DOIs
Publication statusPublished - Sep 1 2010

Keywords

  • Adipose tissue-derived stem cells (hASCs)
  • Bone graft
  • Mineralized extracellular matrix
  • Osteogenic differentiation
  • Trabecular titanium scaffold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

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