Human amnion epithelial cells assemble tenascins and three fibronectin isoforms in the extracellular matrix

Auli Linnala, Enrica Balzac, Luciano Zardi, Ismo Virtanen

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies (MAb) were used to show that cultured human amnion epithelial (HuA) cells produce tenascins (Tn) and isoforms of cellular fibronectin (cFn). Tn polypeptides of Mr 280,000 and 190,000, assembled into extracellular matrix (ECM) but not secreted into the culture medium by HuA cells, were electrophoretically similar to those produced by human fibroblasts as revealed with domain-specific MAbs. The results suggested that most Fn produced by HuA cells contained the extradomain (ED) A and an oncofetal domain but only a minor fraction EDB. In immunofluorescence Tn and Fn were seen in different cytoplasmie granules upon monensin-induced intracellular accumulation. Tn appeared to be deposited in the ECM in colocalization with Fn but distinctly slower. The present results show that cultured normal human epithelial cells synthesize Tn and three isoforms of cFn and secrete them by using different cytoplasmie pathways.

Original languageEnglish
Pages (from-to)74-78
Number of pages5
JournalFEBS Letters
Volume317
Issue number1-2
DOIs
Publication statusPublished - Feb 8 1993

Keywords

  • Amnion epithelial cell
  • Extracellular matrix
  • Fibronectin
  • Secretion
  • Tenascin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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