TY - JOUR
T1 - Human amniotic fluid cells are able to produce IL-6 and IL-8
AU - Mazzucchelli, Iolanda
AU - Avanzini, Maria Antonietta
AU - Ciardelli, Laura
AU - Pagani, Sara
AU - Greco, Rossella
AU - Belloni, Cesare
AU - Castellazzi, Anna Maria
AU - Marconi, Massimo
AU - Rondini, Giorgio
AU - Polatti, Franco
PY - 2004/3
Y1 - 2004/3
N2 - Problem: In order to investigate the role of amniotic fluid cells (AFC) in the establishment of feto-maternal immune relationship, we evaluated their phenotype and capacity to produce cytokines. Methods of Study: CK 7-8, human leukocyte antigen (HLA)-I, HLA-DR, HLA-G, CD1d, CD34, CD45, CD14 surface antigens expression and the intracellular production of IL-4, IL-6, IL-8, IL-10, IL-12, interferon-γ and tumor necrosis factor-α were studied in cultured AFC and in eight samples immediately after amniocentesis using flow cytometry. IL-6 and IL-8 were detected by ELISA in all amniotic fluids and in all culture supernatants. Moreover, IL-6 and IL-8 mRNA were tested in nine samples. Results: Cultured AFC express HLA-I, HLA-G and CK 7-8 and are able to produce IL-6 and IL-8, confirmed by presence of their mRNA. We quantified IL-6 and IL-8 levels in all amniotic fluids and in all supernatants. Conclusion: Surface antigen expression of AFC are not specific of immune cells, but AFC are able to produce cytokines and we can postulate that they may participate in mechanisms involved in normal as well as pathological pregnancy.
AB - Problem: In order to investigate the role of amniotic fluid cells (AFC) in the establishment of feto-maternal immune relationship, we evaluated their phenotype and capacity to produce cytokines. Methods of Study: CK 7-8, human leukocyte antigen (HLA)-I, HLA-DR, HLA-G, CD1d, CD34, CD45, CD14 surface antigens expression and the intracellular production of IL-4, IL-6, IL-8, IL-10, IL-12, interferon-γ and tumor necrosis factor-α were studied in cultured AFC and in eight samples immediately after amniocentesis using flow cytometry. IL-6 and IL-8 were detected by ELISA in all amniotic fluids and in all culture supernatants. Moreover, IL-6 and IL-8 mRNA were tested in nine samples. Results: Cultured AFC express HLA-I, HLA-G and CK 7-8 and are able to produce IL-6 and IL-8, confirmed by presence of their mRNA. We quantified IL-6 and IL-8 levels in all amniotic fluids and in all supernatants. Conclusion: Surface antigen expression of AFC are not specific of immune cells, but AFC are able to produce cytokines and we can postulate that they may participate in mechanisms involved in normal as well as pathological pregnancy.
KW - Amniotic fluid cells
KW - Cytokines
KW - Pregnancy
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M3 - Article
C2 - 15209388
AN - SCOPUS:11144357488
VL - 51
SP - 198
EP - 203
JO - Early pregnancy (Online)
JF - Early pregnancy (Online)
SN - 1046-7408
IS - 3
ER -