Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix

Tullia Maraldi, Massimo Riccio, Elisa Resca, Alessandra Pisciotta, Giovanni B. La Sala, Adriano Ferrari, Giacomo Bruzzesi, Antonella Motta, Claudio Migliaresi, Laura Marzona, Anto De Pol

Research output: Contribution to journalArticlepeer-review


This study investigated the potential of amniotic fluid stem cells (AFSCs) to synthesize mineralized extracellular matrix (ECM) within different porous scaffolds of collagen, poly-d,l-lactic acid (PDLLA), and silk fibroin. The AFSCs were initially differentiated by using an osteogenic medium in two-dimensional culture, and expression of specific bone proteins and the physiologic mineral production by the AFSCs were analyzed. In particular, during differentiation process, AFSCs expressed proteins like Runt-related transcription factor 2 (Runx2), Osterix, Osteopontin, and Osteocalcin with a sequential expression, analogous to those occurring during osteoblast differentiation, and produced extracellular calcium stores. AFSCs were then cultured on three-dimensional (3D) scaffolds and evaluated for their ability to differentiate into osteoblastic cells in vivo. Stem cells were cultured in vitro for 1 week in collagen, fibroin, and PDLLA scaffolds. The effect of predifferentiation of the stem cells in scaffolds on the subsequent bone formation in vivo was determined in a rat subcutaneous model. With the addition of a third dimension, osteogenic differentiation and mineralized ECM production by AFSCs were significantly higher. This study demonstrated the strong potential of AFSCs to produce 3D mineralized bioengineered constructs in vivo and suggests that fibroin may be an effective scaffold material for functional repair of critical size bone defects.

Original languageEnglish
Pages (from-to)2833-2843
Number of pages11
JournalTissue Engineering - Part A
Issue number21-22
Publication statusPublished - Nov 1 2011

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Fingerprint Dive into the research topics of 'Human amniotic fluid stem cells seeded in fibroin scaffold produce in vivo mineralized matrix'. Together they form a unique fingerprint.

Cite this