Human brain endothelial cells and astrocytes produce IL-1β but not IL-10

E. Corsini, A. Dufour, E. Ciusani, M. Gelati, S. Frigerio, A. Gritti, L. Cajola, G. L. Mancardi, G. Massa, A. Salmaggi

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Abstract

The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-α and γ-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1β and β2-microglobulin release were also analysed. IL-10 and TNF-α mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1β and β2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-α was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalScandinavian Journal of Immunology
Volume44
Issue number5
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Immunology

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