Human brain endothelial cells and astrocytes produce IL-1β but not IL-10

E. Corsini, A. Dufour, E. Ciusani, M. Gelati, S. Frigerio, A. Gritti, L. Cajola, G. L. Mancardi, G. Massa, A. Salmaggi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-α and γ-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1β and β2-microglobulin release were also analysed. IL-10 and TNF-α mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1β and β2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-α was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.

Original languageEnglish
Pages (from-to)506-511
Number of pages6
JournalScandinavian Journal of Immunology
Volume44
Issue number5
Publication statusPublished - 1996

Fingerprint

Interleukin-1
Astrocytes
Interleukin-10
Endothelial Cells
Brain
Messenger RNA
Interleukin-2
Human Umbilical Vein Endothelial Cells
Cell Lineage
Multiple Sclerosis
Blood Cells
Down-Regulation
Central Nervous System
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Human brain endothelial cells and astrocytes produce IL-1β but not IL-10. / Corsini, E.; Dufour, A.; Ciusani, E.; Gelati, M.; Frigerio, S.; Gritti, A.; Cajola, L.; Mancardi, G. L.; Massa, G.; Salmaggi, A.

In: Scandinavian Journal of Immunology, Vol. 44, No. 5, 1996, p. 506-511.

Research output: Contribution to journalArticle

Corsini, E. ; Dufour, A. ; Ciusani, E. ; Gelati, M. ; Frigerio, S. ; Gritti, A. ; Cajola, L. ; Mancardi, G. L. ; Massa, G. ; Salmaggi, A. / Human brain endothelial cells and astrocytes produce IL-1β but not IL-10. In: Scandinavian Journal of Immunology. 1996 ; Vol. 44, No. 5. pp. 506-511.
@article{335dc4c2a2d54c10832f1974bb21c60f,
title = "Human brain endothelial cells and astrocytes produce IL-1β but not IL-10",
abstract = "The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-α and γ-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1β and β2-microglobulin release were also analysed. IL-10 and TNF-α mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1β and β2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-α was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.",
author = "E. Corsini and A. Dufour and E. Ciusani and M. Gelati and S. Frigerio and A. Gritti and L. Cajola and Mancardi, {G. L.} and G. Massa and A. Salmaggi",
year = "1996",
language = "English",
volume = "44",
pages = "506--511",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Human brain endothelial cells and astrocytes produce IL-1β but not IL-10

AU - Corsini, E.

AU - Dufour, A.

AU - Ciusani, E.

AU - Gelati, M.

AU - Frigerio, S.

AU - Gritti, A.

AU - Cajola, L.

AU - Mancardi, G. L.

AU - Massa, G.

AU - Salmaggi, A.

PY - 1996

Y1 - 1996

N2 - The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-α and γ-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1β and β2-microglobulin release were also analysed. IL-10 and TNF-α mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1β and β2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-α was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.

AB - The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-α and γ-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1β and β2-microglobulin release were also analysed. IL-10 and TNF-α mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1β and β2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-α was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.

UR - http://www.scopus.com/inward/record.url?scp=10544247775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10544247775&partnerID=8YFLogxK

M3 - Article

C2 - 8947603

AN - SCOPUS:10544247775

VL - 44

SP - 506

EP - 511

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 5

ER -