Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities

Federica Sabatini, Loredana Petecchia, Manuela Tavian, Vanina Jodon De Villeroché, Giovanni A. Rossi, Danièle Brouty-Boyé

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

Mesenchymal stem cells (MSCs) are multipotent cells able to differentiate along different pathways including chondrogenic, osteogenic and adipogenic lineages. MSCs with a fibroblast-like morphology have been identified in human fetal lung. However, their frequency and characterization in human adult lung have not been yet evaluated. Therefore, we analyzed the mesenchymal phenotype and differentiation ability of cultured human adult bronchial fibroblast-like cells (Br) in comparison with those of mesenchymal cell progenitors isolated from fetal lung (ICIG7) and adult bone marrow (BM212) tissues. Surface immunophenotyping by flow cytometry revealed a similar expression pattern of antigens characteristic of marrow-derived MSCs, including CD34 (-), CD45 (-), CD90/Thy-1 (+), CD73/SH3, SH4 (+), CD105/SH2 (+) and CD166/ALCAM (+) in Br, ICIG7 and BM212 cells. There was one exception, STRO-1 antigen, which was only weakly expressed in Br cells. Analysis of cytoskeleton and matrix composition by immunostaining showed that lung and marrow-derived cells homogeneously expressed vimentin and nestin proteins in intermediate filaments while they were all devoid of epithelial cytokeratins. Additionally, α-smooth muscle actin was also present in microfilaments of a low number of cells. All cell types predominantly produced collagen and fibronectin extracellular matrix as evidenced by staining with the monoclonal antibodies to collagen prolyl 4-hydroxylase and fibronectin isoforms containing the extradomain (ED)-A together with ED-B in ICIG7 cells. Br cells similarly to fetal lung and marrow fibroblasts were able to differentiate along the three adipogenic, osteogenic and chondrogenic mesenchymal pathways when cultured under appropriate inducible conditions. Altogether, these data indicate that MSCs are present in human adult lung. They may be actively involved in lung tissue repair under physiological and pathological circumstances.

Original languageEnglish
Pages (from-to)962-971
Number of pages10
JournalLaboratory Investigation
Volume85
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Mesenchymal Stromal Cells
Fibroblasts
Phenotype
Lung
Bone Marrow
Fibronectins
Activated-Leukocyte Cell Adhesion Molecule
Collagen
Prolyl Hydroxylases
Antigens
Nestin
Immunophenotyping
Intermediate Filaments
Vimentin
Keratins
Cytoskeleton
Actin Cytoskeleton
Extracellular Matrix
Smooth Muscle
Actins

Keywords

  • Human fibroblast
  • Lung tissue
  • Mesenchymal differentiation potential
  • Mesenchymal phenotype
  • Mesenchymal stem cell

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities. / Sabatini, Federica; Petecchia, Loredana; Tavian, Manuela; De Villeroché, Vanina Jodon; Rossi, Giovanni A.; Brouty-Boyé, Danièle.

In: Laboratory Investigation, Vol. 85, No. 8, 08.2005, p. 962-971.

Research output: Contribution to journalArticle

Sabatini, Federica ; Petecchia, Loredana ; Tavian, Manuela ; De Villeroché, Vanina Jodon ; Rossi, Giovanni A. ; Brouty-Boyé, Danièle. / Human bronchial fibroblasts exhibit a mesenchymal stem cell phenotype and multilineage differentiating potentialities. In: Laboratory Investigation. 2005 ; Vol. 85, No. 8. pp. 962-971.
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