Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

Tiziano Ingegnere, Francesca Romana Mariotti, Andrea Pelosi, Concetta Quintarelli, Biagio De Angelis, Nicola Tumino, Francesca Besi, Claudia Cantoni, Franco Locatelli, Paola Vacca, Lorenzo Moretta

Research output: Contribution to journalArticlepeer-review

Abstract

CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Original languageEnglish
Pages (from-to)957
JournalFrontiers in Immunology
Volume10
DOIs
Publication statusPublished - 2019

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