TY - JOUR
T1 - Human cardiac and bone marrow stromal cells exhibit distinctive properties related to their origin
AU - Rossini, Alessandra
AU - Frati, Caterina
AU - Lagrasta, Costanza
AU - Graiani, Gallia
AU - Scopece, Alessandro
AU - Cavalli, Stefano
AU - Musso, Ezio
AU - Baccarin, Marco
AU - Di Segni, Marina
AU - Fagnoni, Francesco
AU - Germani, Antonia
AU - Quaini, Eugenio
AU - Mayr, Manuel
AU - Xu, Qingbo
AU - Barbuti, Andrea
AU - Difrancesco, Dario
AU - Pompilio, Giulio
AU - Quaini, Federico
AU - Gaetano, Carlo
AU - Capogrossi, Maurizio C.
PY - 2011/2/15
Y1 - 2011/2/15
N2 - AimsBone marrow mesenchymal stromal cell (BMStC) transplantation into the infarcted heart improves left ventricular function and cardiac remodelling. However, it has been suggested that tissue-specific cells may be better for cardiac repair than cells from other sources. The objective of the present work has been the comparison of in vitro and in vivo properties of adult human cardiac stromal cells (CStC) to those of syngeneic BMStC.Methods and resultsAlthough CStC and BMStC exhibited a similar immunophenotype, their gene, microRNA, and protein expression profiles were remarkably different. Biologically, CStC, compared with BMStC, were less competent in acquiring the adipogenic and osteogenic phenotype but more efficiently expressed cardiovascular markers. When injected into the heart, in rat a model of chronic myocardial infarction, CStC persisted longer within the tissue, migrated into the scar, and differentiated into adult cardiomyocytes better than BMStC.ConclusionOur findings demonstrate that although CStC and BMStC share a common stromal phenotype, CStC present cardiovascular-associated features and may represent an important cell source for more efficient cardiac repair.
AB - AimsBone marrow mesenchymal stromal cell (BMStC) transplantation into the infarcted heart improves left ventricular function and cardiac remodelling. However, it has been suggested that tissue-specific cells may be better for cardiac repair than cells from other sources. The objective of the present work has been the comparison of in vitro and in vivo properties of adult human cardiac stromal cells (CStC) to those of syngeneic BMStC.Methods and resultsAlthough CStC and BMStC exhibited a similar immunophenotype, their gene, microRNA, and protein expression profiles were remarkably different. Biologically, CStC, compared with BMStC, were less competent in acquiring the adipogenic and osteogenic phenotype but more efficiently expressed cardiovascular markers. When injected into the heart, in rat a model of chronic myocardial infarction, CStC persisted longer within the tissue, migrated into the scar, and differentiated into adult cardiomyocytes better than BMStC.ConclusionOur findings demonstrate that although CStC and BMStC share a common stromal phenotype, CStC present cardiovascular-associated features and may represent an important cell source for more efficient cardiac repair.
KW - Cardiac and bone marrow stromal cells
KW - Cardiac regeneration
KW - Cardiovascular differentiation
KW - Myocardial infarction
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U2 - 10.1093/cvr/cvq290
DO - 10.1093/cvr/cvq290
M3 - Article
C2 - 20833652
AN - SCOPUS:78751533852
VL - 89
SP - 650
EP - 660
JO - Cardiovascular Research
JF - Cardiovascular Research
SN - 0008-6363
IS - 3
ER -